Safe Mycobacterium tuberculosis DNA extraction method that does not compromise integrity

Warren R. ; De Kock M. ; Engelke E. ; Myburgh R. ; Van Pittius N.G. ; Victor T. ; Van Helden P. (2006)


Concern has been raised about the efficacy of the heat killing of mycobacteria during the isolation of DNA. We demonstrate a method that allows for the efficient killing of Mycobacterium tuberculosis without compromising DNA integrity for subsequent molecular investigation. This method reduces the risk of infection to the research scientist. Copyright © 2006, American Society for Microbiology. All Rights Reserved.

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