Influence of halogen salts on the production of the ochratoxins by Aspergillus ochraceus Wilh.
Date
2000
Authors
Stander M.A.
Steyn P.S.
Lubben A.
Miljkovic A.
Mantle P.G.
Marais G.J.
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
The first report of the biological production of bromo ochratoxin B by Aspergillus ochraceus Wilh. is presented as well as a study of the influence of potassium bromide, potassium iodide, potassium fluoride, and potassium chloride on the production of ochratoxin A and ochratoxin B. Potassium fluoride and potassium iodide inhibited the growth of the fungus, whereas potassium chloride substantially stimulated the production of ochratoxin A in shaken solid substrate fermentation on whole wheat or shredded wheat, generally giving a high yield of ochratoxins. Increasing levels of potassium bromide led to a decline in ochratoxin A production and an increase in bromo- ochratoxin B, ochratoxin B, and 4-hydroxy ochratoxin B. Nevertheless, A. ochraceus was much less versatile in the bromo analogues than other fungi, which produce metabolites containing chlorine. Analysis included aminopropyl solid-phase extraction column cleanup followed by quantitative analysis on reversed-phase HPLC using fluorescence detection and employing N-(5-chloro-2- hydroxybenzoyl)-phenylalanine as an internal standard.
Description
Keywords
halogen, inorganic salt, ochratoxin, potassium bromide, potassium chloride, potassium fluoride, potassium iodide, article, Aspergillus ochraceus, biosynthesis, chemical structure, chemistry, drug effect, fermentation, fungus growth, metabolism, nonhuman, quantitative assay, reversed phase high performance liquid chromatography, solid phase extraction, Aspergillus ochraceus, Halogens, Ochratoxins, Salts
Citation
Journal of Agricultural and Food Chemistry
48
5
48
5