Degradation of aflatoxin B1 by fungal laccase enzymes

Alberts J.F. ; Gelderblom W.C.A. ; Botha A. ; van Zyl W.H. (2009)


The enzymatic degradation of aflatoxin B1 (AFB1) by white rot fungi through laccase production was investigated in different liquid media. A significant (P < 0.0001) correlation was observed between laccase activity and AFB1 degradation exhibited by representatives of Peniophora and Pleurotus ostreatus cultivated in minimal salts (MSM) (r = 0.93) and mineral salts - malt extract (MSB-MEB) (r = 0.77) liquid media. Peniophora sp. SCC0152 cultured in MSB-MEB liquid medium supplemented with veratryl alcohol and sugarcane bagasse showed high laccase activity (496 U/L), as well as 40.45% AFB1 degradation as monitored using high performance liquid chromatography. P. ostreatus St2-3 cultivated in MSM liquid medium supplemented with veratryl alcohol resulted in laccase activity of 416.39 U/L and 35.90% degradation of AFB1. Aflatoxin B1 was significantly (P < 0.0001) degraded when treated with pure laccase enzyme from Trametes versicolor (1 U/ml, 87.34%) and recombinant laccase produced by Aspergillus niger D15-Lcc2#3 (118 U/L, 55%). Aflatoxin B1 degradation by laccase enzyme from T. versicolor and recombinant laccase enzyme produced by A. niger D15-Lcc2#3 coincided with significant (P < 0.001) loss of mutagenicity of AFB1, as evaluated in the Salmonella typhimurium mutagenicity assay. The degradation of AFB1 by white rot fungi could be an important bio-control measure to reduce the level of this mycotoxin in food commodities. © 2009 Elsevier B.V. All rights reserved.

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