Browsing by Author "Van Dyk, D."
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- ItemCellular Differentiation in Response to Nutrient Availability: The Repressor of Meiosis, Rme1p, Positively Regulates Invasive Growth in Saccharomyces cerevisiae.(GENETICS, 428 EAST PRESTON ST, BALTIMORE, USA, MD, 21202, 2003) Van Dyk, D.; Hansson, G. R.; Pretorius, I. S.; Bauer, Florian
- ItemDivergent regulation of the evolutionarily closely related promoters of the Saccharomyces cerevisiae STA2 and MUC1 genes(1999) Gagiano, M.; Van Dyk, D.; Bauer, Florian; Lambrechts, M. G.; Pretorius, I. S.The 5' upstream regions of the Saccharomyces cerevisiae glucoamylase- encoding genes STA1 to -3 and of the MUC1 (or FLO11) gene, which is critical for pseudohyphal development, invasive growth, and flocculation, are almost identical, and the genes are coregulated to a large extent. Besides representing the largest yeast promoters identified to date, these regions are of particular interest from both a functional and an evolutionary point of view. Transcription of the genes indeed seems to be dependent on numerous transcription factors which integrate the information of a complex network of signaling pathways, while the very limited sequence differences between them should allow the study of promoter evolution on a molecular level. To investigate the transcriptional regulation, we compared the transcription levels conferred by the STA2 and MUC1 promoters under various growth conditions. Our data show that transcription of both genes responded similarly to most environmental signals but also indicated significant divergence in some aspects. We identified distinct areas within the promoters that show specific responses to the activating effect of Flo8p, Msn1p (or Mss10p, Fup1p, or Phd2p), and Mss11p as well as to carbon catabolite repression. We also identified the STA10 repressive effect as the absence of Flo8p, a transcriptional activator of flocculation genes in S. cerevisiae.
- ItemMss11p is a central element of the regulatory network that controls FLO11 expression and invasive growth in Saccharomyces cerevisiae(GENETICS, 428 EAST PRESTON ST, BALTIMORE, USA, MD, 21202, 2004) Van Dyk, D.; Pretorius, I. S.; Bauer, Florian
- ItemThe functional dissection of Mss11p, a transcription factor regulating pseudohyphal differentiation, invasive growth and starch metabolism in Saccharomyces cerevisiae.(BLACKWELL PUBLISHING LTD, 9600 GARSINGTON RD, OXFORD, ENGLAND, OXON, OX4 2DG, 2003) Gagiano, M.; Bauer, Florian; Franken, C. J.; Bester, M. C.; Van Dyk, D.; Pretorius, I. S.