LINE-1 amplification accompanies explosive genome repatterning in rodents

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dc.contributor.authorDobigny G.
dc.contributor.authorOzouf-Costaz C.
dc.contributor.authorWaters P.D.
dc.contributor.authorBonillo C.
dc.contributor.authorCoutanceau J.-P.
dc.contributor.authorVolobouev V.
dc.date.accessioned2011-05-15T15:59:04Z
dc.date.available2011-05-15T15:59:04Z
dc.date.issued2005
dc.description.abstractTransposable elements (TEs) sometimes induce karyotypic changes following recombination, breakage and rearrangement. We used FISH and Southern blot analyses to investigate the amount and distribution of LINE-1 retrotransposons in rodents (genus Taterillus, Muridae, Gerbillinae) that have recently undergone an important genome repatterning. Our results were interpreted in a known phylogenetic framework and clearly showed that LINE-1 elements were greatly amplified and non-randomly distributed in the most rearranged karyotypes. A comparison between FISH and conventional banding patterns provided evidence that LINE-1 insertion sites and chromosome breakpoints were not strongly correlated, thus suggesting that LINE-1 amplification subsequently accompanied Taterillus chromosome evolution. Similar patterns are observed in some cases of genomic stresses (hybrid genomes, cancer and DNA-damaged cells) and usually associated with DNA hypomethylation. We propose that intensively repatterned genomes face transient stress phases during which some epigenetic features, such as DNA methylation, are relaxed, thus allowing TE amplification. © 2004 Kluwer Academic Publishers.
dc.description.versionArticle
dc.identifier.citationChromosome Research
dc.identifier.citation12
dc.identifier.citation8
dc.identifier.issn9673849
dc.identifier.other10.1007/s10577-005-5265-y
dc.identifier.urihttp://hdl.handle.net/10019.1/10989
dc.subjectarticle
dc.subjectcancer
dc.subjectchromosome band
dc.subjectchromosome breakage
dc.subjectcorrelation analysis
dc.subjectDNA damage
dc.subjectDNA methylation
dc.subjectevolution
dc.subjectfluorescence in situ hybridization
dc.subjectgene amplification
dc.subjectgerbil
dc.subjecthybrid gene
dc.subjectkaryotype
dc.subjectMuridae
dc.subjectphylogeny
dc.subjectpriority journal
dc.subjectretroposon
dc.subjectrodent
dc.subjectSouthern blotting
dc.subjectstress
dc.subjectAnimals
dc.subjectBlotting, Southern
dc.subjectChromosomes, Mammalian
dc.subjectDNA Transposable Elements
dc.subjectGenome
dc.subjectGerbillinae
dc.subjectIn Situ Hybridization, Fluorescence
dc.subjectKaryotyping
dc.subjectLong Interspersed Nucleotide Elements
dc.subjectNucleic Acid Amplification Techniques
dc.subjectPhylogeny
dc.titleLINE-1 amplification accompanies explosive genome repatterning in rodents
dc.typeArticle
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