A recently evolved sublineage of the Mycobacterium tuberculosis Beijing strain family is associated with an increased ability to spread and cause disease

Simple item page
dc.contributor.authorHanekom M.
dc.contributor.authorVan Der Spuy G.D.
dc.contributor.authorStreicher E.
dc.contributor.authorNdabambi S.L.
dc.contributor.authorMcEvoy C.R.E.
dc.contributor.authorKidd M.
dc.contributor.authorBeyers N.
dc.contributor.authorVictor T.C.
dc.contributor.authorVan Helden P.D.
dc.contributor.authorWarren R.M.
dc.date.accessioned2011-05-15T15:56:55Z
dc.date.available2011-05-15T15:56:55Z
dc.date.issued2007
dc.description.abstractThis study aimed to reconstruct the evolutionary history of Beijing strains of Mycobacterium tuberculosis and to test the hypothesis that evolution has influenced the ability of the Beijing strains within the different Beijing sublineages to spread and cause disease. A PCR-based method was used to analyze the genome structure of 40 different loci in 325 Beijing isolates collected from new and retreatment tuberculosis patients from an urban setting and 270 Beijing isolates collected from high-risk tuberculosis patients from a rural setting in the Western Cape, South Africa. The resulting data were subjected to phylogenetic analysis using the neighbor joining algorithm. Phylogenetic reconstructions were highly congruent with the "gold standard" phylogenetic tree based on synonymous single-nucleotide polymorphisms, thereby allowing a prediction of the order in which the evolutionary events had occurred. A total of seven independently evolving Beijing sublineages were identified. Analysis of epidemiological data in relation to the Beijing sublineage suggested an association between recent evolutionary change and frequency of occurrence in an urban population (P < 0.001) as well as in the rural population (P < 0.001). This concept was further supported by an association between more recently evolved Beijing strains and an increased ability to transmit and to cause disease (odds ratio, 5.82; 95% confidence interval, 3.13 to 10.82 [P < 0.001]). An association between Beijing sublineage and demographic and clinical parameters and drug resistance could not be demonstrated. From these data, we suggest that the pathogenic characteristics of Beijing strains are not conserved but rather that strains within individual lineages have evolved unique pathogenic characteristics. Copyright © 2007, American Society for Microbiology. All Rights Reserved.
dc.description.versionArticle
dc.identifier.citationJournal of Clinical Microbiology
dc.identifier.citation45
dc.identifier.citation5
dc.identifier.issn951137
dc.identifier.other10.1128/JCM.02191-06
dc.identifier.urihttp://hdl.handle.net/10019.1/10113
dc.subjectisoniazid
dc.subjectrifampicin
dc.subjectarticle
dc.subjectbacterial strain
dc.subjectbacterial transmission
dc.subjectbacterium isolate
dc.subjectChina
dc.subjectcontrolled study
dc.subjectdisease course
dc.subjectgene identification
dc.subjectgenetic analysis
dc.subjectgenetic association
dc.subjectgenetic variability
dc.subjecthigh risk patient
dc.subjecthuman
dc.subjecthuman tissue
dc.subjectMycobacterium tuberculosis
dc.subjectnonhuman
dc.subjectnucleotide sequence
dc.subjectphylogenetic tree construction method
dc.subjectphylogeny
dc.subjectpolymerase chain reaction
dc.subjectprediction
dc.subjectpriority journal
dc.subjectrural area
dc.subjectsingle nucleotide polymorphism
dc.subjectSouth Africa
dc.subjecttuberculosis
dc.subjecturban area
dc.subjectAntitubercular Agents
dc.subjectEvolution
dc.subjectGenotype
dc.subjectHumans
dc.subjectMycobacterium tuberculosis
dc.subjectSouth Africa
dc.subjectTuberculosis
dc.subjectVariation (Genetics)
dc.subjectVirulence
dc.subjectMycobacterium tuberculosis
dc.titleA recently evolved sublineage of the Mycobacterium tuberculosis Beijing strain family is associated with an increased ability to spread and cause disease
dc.typeArticle
Files
Collections
Stellenbosch University - Scopus Publications