Leucocytes and intrinsic ROS production may be factors compromising sperm chromatin condensation status

Simple item page
dc.contributor.authorHenkel R.
dc.contributor.authorBastiaan H.S.
dc.contributor.authorSchuller S.
dc.contributor.authorHoppe I.
dc.contributor.authorStarker W.
dc.contributor.authorMenkveld R.
dc.date.accessioned2011-05-15T16:15:24Z
dc.date.available2011-05-15T16:15:24Z
dc.date.issued2010
dc.description.abstractConsidering that the final protection of the DNA against major assaults in terms of chromatin condensation is finalised in the epididymis, it is not known how sperm production of reactive oxygen species (ROS) and inflammatory processes can contribute to protamine deficiency that is predetermined in the testes. Therefore, this study aimed at investigating relationships between poor chromatin condensation, morphology, ROS production, DNA damage and the impact of the presence of leucocytes. In 70 patients, sperm DNA status was determined using TUNEL and chromomycin A3 (CMA3) assays, and ROS-production by means of dihydroethidine. Morphology was evaluated according to strict criteria. The percentage of CMA3-positive spermatozoa and leucocyte concentration (r = 0.178, P = 0.0377) as well as percentage of ROS-positive spermatozoa (r = 0.3010; P = 0.012) correlated significantly. Particularly, patients with leucocyte counts >0.5 × 106 ml-1 exhibited higher CMA3 positivity. No association was found between CMA3 positivity, TUNEL positivity and sperm morphology. While P- (poor prognosis: 0-4% normal morphology) and G-pattern (good prognosis: 5-14% normal morphology) morphology did not differ regarding chromatin condensation, P-pattern patients had a significantly higher percentage of DNA fragmentation (P = 0.0323). As oxidative stress is associated with disturbed chromatin condensation, results suggest that the idea that under-protamination of sperm DNA will automatically lead to DNA fragmentation might have to be revisited. © 2010 Blackwell Verlag GmbH.
dc.description.versionArticle
dc.identifier.citationAndrologia
dc.identifier.citation42
dc.identifier.citation2
dc.identifier.issn03034569
dc.identifier.other10.1111/j.1439-0272.2009.00967.x
dc.identifier.urihttp://hdl.handle.net/10019.1/13321
dc.subjectchromomycin A3
dc.subjectreactive oxygen metabolite
dc.subjectadult
dc.subjectarticle
dc.subjectcell structure
dc.subjectchromatin condensation
dc.subjectcontrolled study
dc.subjectDNA damage
dc.subjectDNA fragmentation
dc.subjecthuman
dc.subjectleukocyte
dc.subjectleukocyte count
dc.subjectmajor clinical study
dc.subjectmale
dc.subjectnick end labeling
dc.subjectoxidative stress
dc.subjectprognosis
dc.subjectprospective study
dc.subjectsperm
dc.subjectspermatozoon
dc.subjectspermatozoon motility
dc.subjectAdult
dc.subjectChromatin
dc.subjectChromatin Assembly and Disassembly
dc.subjectDNA Fragmentation
dc.subjectHumans
dc.subjectLeukocytes
dc.subjectMale
dc.subjectMiddle Aged
dc.subjectReactive Oxygen Species
dc.subjectSpermatozoa
dc.titleLeucocytes and intrinsic ROS production may be factors compromising sperm chromatin condensation status
dc.typeArticle
Files
Collections
Stellenbosch University - Scopus Tygerberg Hospital Publications