The inhibitory effect of acetaminophen on the myeloperoxidase-induced antimicrobial system of the polymorphonuclear leukocyte

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dc.contributor.authorVan Zyl J.M.
dc.contributor.authorBasson K.
dc.contributor.authorVan der Walt B.J.
dc.date.accessioned2011-05-15T16:01:18Z
dc.date.available2011-05-15T16:01:18Z
dc.date.issued1989
dc.description.abstractAcetaminophen binds via its acetamido side chain to purified myeloperoxidase in a pH-dependent manner and maximum binding occurred around pH 6. The H2O2-dependent myeloperoxidase-catalysed polymerization products of acetaminophen had excitation maxima at 304 nm and 334 nm in acid and alkaline solutions, respectively, and an intense blue fluorescence maximum at 426 nm. Acetaminophen can compete effectively with Cl- as myeloperoxidase substrate and thus HOCl formation is suppressed while HOCl, nevertheless present, can be scavenged by the drug. In this way the microbicidal action of the myeloperoxidase-H2O2-Cl- system can be seriously limited in the presence of high concentrations of acetaminophen. To study the effect of acetaminophen on peptide bond splitting in the myeloperoxidase antimicrobial system, thyroglobulin was used as a model peptide. Peptide bond splitting was inhibited at acetaminophen concentrations below the accepted toxic range for plasma values.
dc.description.versionArticle
dc.identifier.citationBiochemical Pharmacology
dc.identifier.citation38
dc.identifier.citation1
dc.identifier.issn62952
dc.identifier.other10.1016/0006-2952(89)90163-9
dc.identifier.urihttp://hdl.handle.net/10019.1/11912
dc.subjectmyeloperoxidase
dc.subjectparacetamol
dc.subjectthyroglobulin
dc.subjectbactericidal activity
dc.subjecthuman
dc.subjecthuman cell
dc.subjectneutrophil
dc.subjectpriority journal
dc.subjectAcetaminophen
dc.subjectBlood Bactericidal Activity
dc.subjectHuman
dc.subjectHydrogen-Ion Concentration
dc.subjectHypochlorous Acid
dc.subjectNeutrophils
dc.subjectPeroxidase
dc.subjectSupport, Non-U.S. Gov't
dc.subjectThyroglobulin
dc.titleThe inhibitory effect of acetaminophen on the myeloperoxidase-induced antimicrobial system of the polymorphonuclear leukocyte
dc.typeArticle
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