OAS1, OAS2 and OAS3 restrict intracellular M. tb replication and enhance cytokine secretion
Date
2019
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier
Abstract
The 2′,5′ (OASs) are known as mediators of the antiviral response system through activation of the RNA cleavage pathway. Interestingly, we observe OAS1 , OAS2 and OAS3 upregulation in a number of gene expression signatures which discriminate active TB from latent TB infection, however their biological role during bacterial infection has not yet been elucidated. We observed that the expression of these genes was associated with pathogenicity and virulence of mycobacteria as infection with Mycobacterium bovis BCG failed to significantly induce OAS expression. Further, we observed that after silencing of these genes, M. tb CFU counts increased significantly 96 h post-infection in comparison to the respective controls. Luminex revealed that OAS silencing significantly decreased IL-1β, TNF-α and MCP-1 and had no effect of IL-10 secretion. We show for the first time that OAS1, 2 and 3 restrict intracellular pathogenic mycobacterial replication and enhance pro-inflammatory cytokine secretion.
Description
CITATION: Leisching, G., et al. 2019. OAS1, OAS2 and OAS3 restrict intracellular M. tb replication and enhance cytokine secretion. International Journal of Infectious Diseases, 80:S77-S84, doi:10.1016/j.ijid.2019.02.029.
The original publication is available at https://www.clinicalkey.com/
The original publication is available at https://www.clinicalkey.com/
Keywords
Citation
Leisching, G., et al. 2019. OAS1, OAS2 and OAS3 restrict intracellular M. tb replication and enhance cytokine secretion. International Journal of Infectious Diseases, 80:S77-S84, doi:10.1016/j.ijid.2019.02.029