Stable transformation of Hi-II maize using the particle inflow gun

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dc.contributor.authorO'Kennedy M.M.
dc.contributor.authorBurger J.T.
dc.contributor.authorWatson T.G.
dc.date.accessioned2011-05-15T15:55:21Z
dc.date.available2011-05-15T15:55:21Z
dc.date.issued1998
dc.description.abstractEmbryogenic type II callus, initiated from cultured immature zygotic embryos of the maize line Hi-II, was transformed by microprojectile bombardment using a particle inflow gun. This highly regenerable maize line was transformed with the plasmid pAHC25, a dual expression vector containing the uidA reporter gene and the bar selectable marker gene, both under the control of the maize ubiquitin promoter. Transgenic plants were obtained from 10 independent transformation events. Transmission of the bar and uidA genes to the progeny of three of these lines was demonstrated by Southern blot analysis and by transgene expression.
dc.description.versionArticle
dc.identifier.citationSouth African Journal of Science
dc.identifier.citation94
dc.identifier.citation4
dc.identifier.issn382353
dc.identifier.urihttp://hdl.handle.net/10019.1/9728
dc.subjectcallus culture
dc.subjectembryo development
dc.subjectexpression vector
dc.subjectgene expression
dc.subjectmaize
dc.subjectmicroprojectile bombardment
dc.subjectplasmid
dc.subjectregenerative ability
dc.subjectreporter gene
dc.subjectsouthern blotting
dc.subjecttransgenic plant
dc.titleStable transformation of Hi-II maize using the particle inflow gun
dc.typeArticle
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