Potential of novel Mycobacterium tuberculosis infection phase-dependent antigens in the diagnosis of TB disease in a high burden setting

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dc.contributor.authorChegou, Novel N.en_ZA
dc.contributor.authorBlack, Gillian F.en_ZA
dc.contributor.authorLoxton, Andre G.en_ZA
dc.contributor.authorStanley, Kim
dc.contributor.authorEssone, Paulin N.en_ZA
dc.contributor.authorKlein, Michel R.en_ZA
dc.contributor.authorParida, Shreemanta K.en_ZA
dc.contributor.authorKaufmann, Stefan H. E.en_ZA
dc.contributor.authorDoherty, T. Marken_ZA
dc.contributor.authorFriggen, Annemieke H.en_ZA
dc.contributor.authorFranken, Kees L.en_ZA
dc.contributor.authorOttenhoff, Tom H.en_ZA
dc.contributor.authorWalzl, Gerharden_ZA
dc.date.accessioned2012-05-17T14:26:20Z
dc.date.available2012-05-17T14:26:20Z
dc.date.issued2012-01
dc.date.updated2012-02-21T06:33:07Z
dc.descriptionThe original publication is available at http://www.biomedcentral.com/1471-2334/12/10en_ZA
dc.descriptionPublication of this article was funded by the Stellenbosch University Open Access Fund.
dc.description.abstractAbstract Background Confirming tuberculosis (TB) disease in suspects in resource limited settings is challenging and calls for the development of more suitable diagnostic tools. Different Mycobacterium tuberculosis (M.tb) infection phase-dependent antigens may be differentially recognized in infected and diseased individuals and therefore useful as diagnostic tools for differentiating between M.tb infection states. In this study, we assessed the diagnostic potential of 118 different M.tb infection phase-dependent antigens in TB patients and household contacts (HHCs) in a high-burden setting. Methods Antigens were evaluated using the 7-day whole blood culture technique in 23 pulmonary TB patients and in 19 to 21 HHCs (total n = 101), who were recruited from a high-TB incidence community in Cape Town, South Africa. Interferon-gamma (IFN-γ) levels in culture supernatants were determined by ELISA. Results Eight classical TB vaccine candidate antigens, 51 DosR regulon encoded antigens, 23 TB reactivation antigens, 5 TB resuscitation promoting factors (rpfs), 6 starvation and 24 other stress response-associated TB antigens were evaluated in the study. The most promising antigens for ascertaining active TB were the rpfs (Rv0867c, Rv2389c, Rv2450c, Rv1009 and Rv1884c), with Areas under the receiver operating characteristics curves (AUCs) between 0.72 and 0.80. A combination of M.tb specific ESAT-6/CFP-10 fusion protein, Rv2624c and Rv0867c accurately predicted 73% of the TB patients and 80% of the non-TB cases after cross validation. Conclusions IFN-γ responses to TB rpfs show promise as TB diagnostic candidates and should be evaluated further for discrimination between M.tb infection states.en_ZA
dc.description.versionPublishers' Versionen_ZA
dc.format.extent14 p. : ill.
dc.identifier.citationChegou, N. > et al. 2012. Potential of novel Mycobacterium tuberculosis infection phase-dependent antigens in the diagnosis of TB disease in a high burden setting. BMC Infectious Diseases, 12(1):10.en_ZA
dc.identifier.urihttp://dx.doi.org/10.1186/1471-2334-12-10
dc.identifier.urihttp://hdl.handle.net/10019.1/21116
dc.language.isoen_ZAen_ZA
dc.language.rfc3066en
dc.publisherBioMed Centralen_ZA
dc.rights.holderChegou et al.; licensee BioMed Central Ltd.en_ZA
dc.subjectElisaen_ZA
dc.subjectAntigensen_ZA
dc.subjectMycobacterial diseases -- Diagnosis -- South Africa -- Cape Townen_ZA
dc.subjectMycobacterial antigens -- South Africa -- Researchen_ZA
dc.subjectTuberculosis -- Diagnosis, Noninvasiveen_ZA
dc.subject.lcshMycobacterium tuberculosisen_ZA
dc.subject.lcshTuberculosis Diagnosis -- South Africa -- Cape Townen_ZA
dc.subject.lcshDiagnosis, Laboratoryen_ZA
dc.titlePotential of novel Mycobacterium tuberculosis infection phase-dependent antigens in the diagnosis of TB disease in a high burden settingen_ZA
dc.typeArticleen_ZA
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