Phosphatidylcholine biosynthesis in myocardial ischaemia

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dc.contributor.authorLochner A.
dc.contributor.authorde Villiers M.
dc.date.accessioned2011-05-15T15:59:44Z
dc.date.available2011-05-15T15:59:44Z
dc.date.issued1989
dc.description.abstractIn this study the effect of myocardial ischaemia was evaluated on two aspects of phospholipid metabolism: (i) the de novo synthesis of myocardial phospholipids, as indicated by the incorporation of (methyl-3H) choline and (ii) the incorporation of radiolabelled long chain fatty acids into tissue phospholipids. Two models of ischaemia were used namely normothermic ischaemic arrest and hypoxic, low-flow perfusion of the isolated rat heart. The results showed that within 10 min, hypoxic low-flow perfusion significantly inhibited the incorporation rate of (methyl-3H)choline into tissue phospholipids. Since the tissue choline content remained unaltered under these conditions, the results suggested that the de novo synthesis of phosphatidylcholine is very susceptible to ischaemic damage. Inhibition of (methyl-3H)choline incorporation into tissue phospholipids appeared to be due to both a reduction in choline uptake and specific inhibition of the CDP pathway. Perfusion with glucose (10 mM) as substrate completely abolished the ischaemia-induced reduction in (methyl-3H)choline incorporation, indicating that glycolytically produced ATP played an important role in phosphatidylcholine biosynthesis. In contrast to these results, myocardial ischaemia stimulated the incorporation of long-chain saturated and unsaturated fatty acids into tissue phospholipids. In summary, the results obtained showed that myocardial ischaemia profoundly affected phospholipid metabolism which, in turn, might contribute to membrane damage.
dc.description.versionArticle
dc.identifier.citationJournal of Molecular and Cellular Cardiology
dc.identifier.citation21
dc.identifier.citation2
dc.identifier.issn222828
dc.identifier.urihttp://hdl.handle.net/10019.1/11332
dc.subjectadenosine triphosphate
dc.subjectcholine
dc.subjectcytidine diphosphate
dc.subjectphosphatidylcholine
dc.subjectphospholipid
dc.subjectradioisotope
dc.subjectanimal cell
dc.subjectheart
dc.subjectischemia
dc.subjectnonhuman
dc.subjectpriority journal
dc.subjectrat
dc.subjectAdenosine Triphosphate
dc.subjectAnimal
dc.subjectAnoxia
dc.subjectCholine
dc.subjectCholine Kinase
dc.subjectCholine-Phosphate Cytidylyltransferase
dc.subjectCoronary Disease
dc.subjectCytidine Triphosphate
dc.subjectFatty Acids
dc.subjectMale
dc.subjectMyocardium
dc.subjectNucleotidyltransferases
dc.subjectPhosphatidylcholines
dc.subjectPhospholipids
dc.subjectRats
dc.subjectRats, Inbred Strains
dc.titlePhosphatidylcholine biosynthesis in myocardial ischaemia
dc.typeArticle
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