Molecular characterization of Staphylococcus aureus isolates from various healthcare institutions in Nairobi, Kenya : a cross sectional study

Simple item page
dc.contributor.authorOmuse, Geoffreyen_ZA
dc.contributor.authorVan Zyl, Kristien Nel.en_ZA
dc.contributor.authorHoek, Kimen_ZA
dc.contributor.authorAbdulgader, Shimaen_ZA
dc.contributor.authorKariuki, Samuelen_ZA
dc.contributor.authorWhitelaw, Andrewen_ZA
dc.contributor.authorRevathi, Gunturuen_ZA
dc.date.accessioned2017-01-24T10:19:27Z
dc.date.available2017-01-24T10:19:27Z
dc.date.issued2016-09-20
dc.date.updated2016-12-09T12:07:58Z
dc.descriptionCITATION: Omuse, G., et al. 2016. Molecular characterization of Staphylococcus aureus isolates from various healthcare institutions in Nairobi, Kenya : a cross sectional study. Annals of Clinical Microbiology and Antimicrobials, 15:51, doi:10.1186/s12941-016-0171-z.
dc.descriptionThe original publication is available at http://ann-clinmicrob.biomedcentral.com
dc.description.abstractBackground: Staphylococcus aureus (S. aureus) has established itself over the years as a major cause of morbidity and mortality both within the community and in healthcare settings. Methicillin resistant S. aureus (MRSA) in particular has been a major cause of nosocomial infections resulting in significant increase in healthcare costs. In Africa, the MRSA prevalence has been shown to vary across different countries. In order to better understand the epidemiology of MRSA in a setting, it is important to define its population structure using molecular tools as different clones have been found to predominate in certain geographical locations. Methods: We carried out PFGE, MLST, SCCmec and spa typing of selected S. aureus isolates from a private and public referral hospital in Nairobi, Kenya. Results: A total of 93 S. aureus isolates were grouped into 19 PFGE clonal complexes (A–S) and 12 singletons. From these, 55 (32 MRSA and 23 MSSA) representative isolates from each PFGE clonal complex and all singletons were spa typed. There were 18 different MRSA spa types and 22 MSSA spa types. The predominant MRSA spa type was t037 comprising 40.6 % (13/32) of all MRSA. In contrast, the MSSA were quite heterogeneous, only 2 out of 23 MSSA shared the same spa type. Two new MRSA spa types (t13149 and t13150) and 3 new MSSA spa types (t13182, t13193 and t13194) were identified. The predominant clonal complex was CC 5 which included multi-locus sequence types 1, 8 and 241. Conclusion: In contrast to previous studies published from Kenya, there’s marked genetic diversity amongst clinical MRSA isolates in Nairobi including the presence of well-known epidemic MRSA clones. Given that these clones are resident within our referral hospitals, adherence to strict infection control measures needs to be ensured to reduce morbidity and mortality associated with hospital acquired MRSA infections.en_ZA
dc.description.urihttp://ann-clinmicrob.biomedcentral.com/articles/10.1186/s12941-016-0171-z
dc.description.versionPublisher's version
dc.format.extent9 pages
dc.identifier.citationOmuse, G., et al. 2016. Molecular characterization of Staphylococcus aureus isolates from various healthcare institutions in Nairobi, Kenya : a cross sectional study. Annals of Clinical Microbiology and Antimicrobials, 15:51, doi:10.1186/s12941-016-0171-z
dc.identifier.issn1476-0711(Online)
dc.identifier.otherdoi:10.1186/s12941-016-0171-z
dc.identifier.urihttp://hdl.handle.net/10019.1/100507
dc.language.isoen_ZAen_ZA
dc.publisherBioMed Central
dc.rights.holderAuthor retains copyright
dc.subjectStaphylococcus aureus infections -- Kenia -- Nairobien_ZA
dc.subjectStaphylococcus aureus -- Epidemiologyen_ZA
dc.subjectStaphylococcus aureus -- Molecular aspectsen_ZA
dc.subjectNosocomial infectionsen_ZA
dc.subjectHospital infectionsen_ZA
dc.subjectMethicillin resistanceen_ZA
dc.titleMolecular characterization of Staphylococcus aureus isolates from various healthcare institutions in Nairobi, Kenya : a cross sectional studyen_ZA
dc.typeArticle
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
omuse_molecular_2016.pdf
Size:
1.12 MB
Format:
Adobe Portable Document Format
Description:
Download article
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.95 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Research Articles (Medical Microbiology)