Cytotoxic activity of crude extracts from Datura stramonium’s fungal endophytes against A549 lung carcinoma and UMG87 glioblastoma cell lines and LC-QTOF-MS/MS based metabolite profiling

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dc.contributor.authorTapfuma, Kudzanai Ianen_ZA
dc.contributor.authorUche-Okereafor, Nkemdinmaen_ZA
dc.contributor.authorSebola, Tendani Edithen_ZA
dc.contributor.authorHussan, Raeesaen_ZA
dc.contributor.authorMekuto, Lukhanyoen_ZA
dc.contributor.authorMakatini, Maya Mellisaen_ZA
dc.contributor.authorGreen, Ezekielen_ZA
dc.contributor.authorMavumengwana, Vuyoen_ZA
dc.date.accessioned2019-11-25T06:40:03Z
dc.date.available2019-11-25T06:40:03Z
dc.date.issued2019-11-21
dc.date.updated2019-11-24T04:20:06Z
dc.descriptionCITATION: Tapfuma, K. I., et al. 2019. Cytotoxic activity of crude extracts from Datura stramonium’s fungal endophytes against A549 lung carcinoma and UMG87 glioblastoma cell lines and LC-QTOF-MS/MS based metabolite profiling. BMC Complementary and Alternative Medicine, 19:330, doi:10.1186/s12906-019-2752-9.
dc.descriptionThe original publication is available at https://bmccomplementalternmed.biomedcentral.com
dc.description.abstractBackground: Endophytic fungi are a proven source of bioactive secondary metabolites that may provide lead compounds for novel drug discovery. In this study, crude extracts from fungal endophytes isolated from Datura stramonium were evaluated for cytotoxic activity on two human cancer cell lines. Methods: Fungal endophytes were isolated from surface sterilized aerial parts of D. stramonium and identified using molecular, morphological and phylogenetic methods. Ethyl acetate crude extracts from these isolates were evaluated for cytotoxic activity on A549 lung carcinoma and UMG87 glioblastoma cell lines. Metabolite profiling was then performed by liquid chromatography coupled to quadrupole time-of-flight with tandem mass spectrometry (LC-QTOF-MS/MS) for the cytotoxic crude extract. Results: Eleven fungal endophytes were identified from D. stramonium. Significant cytotoxicity was only observed from the crude extract of Alternaria sp. KTDL7 on UMG87 glioblastoma cells (IC50 = 21.49 μg/ml). Metabolite profiling of this crude extract tentatively revealed the presence of the following secondary metabolites: 1,8-dihydroxynaphthalene (1), anserinone B (2), phelligridin B (3), metacytofilin (4), phomopsidin (5) and vermixocin A (6). Compounds 2 and 3 have been shown to be cytotoxic in literature. Conclusion: The findings in this study suggest that the crude extract of Alternaria sp. KTDL7 possesses compound(s) cytotoxic to glioblastoma multiforme cells. Future studies to isolate and characterize the cytotoxic compound(s) from this fungus could result in lead development of a fungal-based drug for glioblastoma multiforme treatment.
dc.description.urihttps://bmccomplementalternmed.biomedcentral.com/articles/10.1186/s12906-019-2752-9
dc.description.versionPublisher's version
dc.format.extent12 pages
dc.identifier.citationTapfuma, K. I., et al. 2019. Cytotoxic activity of crude extracts from Datura stramonium’s fungal endophytes against A549 lung carcinoma and UMG87 glioblastoma cell lines and LC-QTOF-MS/MS based metabolite profiling. BMC Complementary and Alternative Medicine, 19:330, doi:10.1186/s12906-019-2752-9
dc.identifier.issn1472-6882 (online)
dc.identifier.otherdoi:10.1186/s12906-019-2752-9
dc.identifier.urihttp://hdl.handle.net/10019.1/106870
dc.language.isoen_ZAen_ZA
dc.publisherBMC (part of Springer Nature)
dc.rights.holderAuthors retain copyright
dc.subjectDatura stramonium
dc.subjectEndophytesen_ZA
dc.subjectSecondary metabolitesen_ZA
dc.subjectCarcinomaen_ZA
dc.subjectLung canceren_ZA
dc.subjectAntineoplastic agentsen_ZA
dc.titleCytotoxic activity of crude extracts from Datura stramonium’s fungal endophytes against A549 lung carcinoma and UMG87 glioblastoma cell lines and LC-QTOF-MS/MS based metabolite profilingen_ZA
dc.typeArticle
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