Selectivity of hyaluronic acid binding for spermatozoa with normal Tygerberg strict morphology

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dc.contributor.authorPrinosilova P.
dc.contributor.authorKruger T.
dc.contributor.authorSati L.
dc.contributor.authorOzkavukcu S.
dc.contributor.authorVigue L.
dc.contributor.authorKovanci E.
dc.contributor.authorHuszar G.
dc.date.accessioned2011-05-15T16:17:48Z
dc.date.available2011-05-15T16:17:48Z
dc.date.issued2009
dc.description.abstractDuring spermiogenesis, a plasma membrane remodelling step facilitates formation of sperm zona pellucida and hyaluronic acid (HA) binding sites. Enrichment of Tygerberg normal spermatozoa in HA-bound versus semen sperm fractions was postulated. Semen was placed on the uncoated A side and HA-coated B side of a semen chamber. After 15 min, the HA binding score (proportion of HA-bound motile spermatozoa) was assessed on the B side, and unbound spermatozoa were removed by gentle rinsing. Following Diff-Quick staining, sperm morphology of A and B sides was evaluated by three blinded investigators at Yale and Tygerberg. The proportion of Tygerberg normal spermatozoa was higher in HA-bound versus semen spermatozoa (n = 63 subjects) with a 3.04-fold improvement (95% confidence limits: 1.9-4.7) in 37 teratozoospermic men, comparable with a 4.2-fold enrichment in zona pellucida-bound spermatozoa reported earlier by the Tygerberg group. The morphology scores of three investigators were different but related, indicating that the variations reflect individual-to-individual differences in the perception of shape normality. The selection power of HA and zona pellucida for normal spermatozoa are similar. The sperm biomarkers of creatine phosphokinase (reflecting retained cytoplasm in arrested maturity spermatozoa) and chaperone protein HspA2 (heat shock protein) were proportional with sperm HA binding. As HA binding reflects sperm maturity and function, the combination of Tygerberg morphology and HA binding is likely to improve male infertility management. © 2009 Published by Reproductive Healthcare Ltd.
dc.description.versionArticle
dc.identifier.citationReproductive BioMedicine Online
dc.identifier.citation18
dc.identifier.citation2
dc.identifier.issn14726483
dc.identifier.urihttp://hdl.handle.net/10019.1/14381
dc.subjectcreatine kinase
dc.subjectheat shock protein
dc.subjecthyaluronic acid
dc.subjectarticle
dc.subjectazoospermia
dc.subjectbinding kinetics
dc.subjectbinding site
dc.subjectcell membrane
dc.subjectcontrolled study
dc.subjectcytoplasm
dc.subjecthistochemistry
dc.subjecthuman
dc.subjecthuman cell
dc.subjectmale
dc.subjectmale infertility
dc.subjectmorphology
dc.subjectspermatogenesis
dc.subjectspermatozoon maturation
dc.subjectspermatozoon motility
dc.subjectzona pellucida
dc.subjectBiological Markers
dc.subjectCytological Techniques
dc.subjectHumans
dc.subjectHyaluronic Acid
dc.subjectMale
dc.subjectQuality Control
dc.subjectResearch Design
dc.subjectSperm Maturation
dc.subjectSperm Motility
dc.subjectSpermatozoa
dc.subjectSubstrate Specificity
dc.titleSelectivity of hyaluronic acid binding for spermatozoa with normal Tygerberg strict morphology
dc.typeArticle
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