High-density spore production of a B. cereus aquaculture biological agent by nutrient supplementation

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dc.contributor.authorLalloo R.
dc.contributor.authorMaharajh D.
dc.contributor.authorGorgens J.
dc.contributor.authorGardiner N.
dc.contributor.authorGorgens J.F.
dc.date.accessioned2011-05-15T15:56:36Z
dc.date.available2011-05-15T15:56:36Z
dc.date.issued2009
dc.description.abstractPrevious studies have demonstrated the efficacy of our Bacillus cereus isolate (NRRL 100132) in reducing concentrations of nitrogenous wastes and inhibiting growth of fish pathogens. In vivo efficacy and tolerance to a range of physiological conditions in systems used to rear Cyprinus carpio make this isolate an excellent candidate for aquaculture applications. Production cost is an important consideration in development of commercially relevant biological products, and this study examines the optimization of nutrient supplementation, which has an impact on high-density production of spores by fermentation. Corn steep liquor (CSL) was identified as a lower cost and more effective nutrient source in comparison to conventional nutrient substrates, in particular yeast extract and nutrient broth. The improved sporulation performance of B. cereus could be related to the increased availability of free amino acids, carbohydrates, and minerals in CSL, which had a positive effect on sporulation efficiency. The impact of nutrient concentration on spore yield and productivity was modeled to develop a tool for optimization of nutrient concentration in fermentation. An excellent fit of the model was confirmed in laboratory fermentation studies. A cost comparison revealed that production using liquid phytase and ultrafiltered-treated CSL was less expensive than spray-dried CSL and supported cultivation of B. cereus spores at densities higher than 1∈×∈1010 CFU ml-1. © 2009 Springer-Verlag.
dc.description.versionArticle
dc.identifier.citationApplied Microbiology and Biotechnology
dc.identifier.citation83
dc.identifier.citation1
dc.identifier.issn1757598
dc.identifier.other10.1007/s00253-008-1845-z
dc.identifier.urihttp://hdl.handle.net/10019.1/9937
dc.subjectBacillus cereus
dc.subjectBiological agent
dc.subjectBiological products
dc.subjectCorn steep liquor
dc.subjectCost comparisons
dc.subjectCyprinus carpio
dc.subjectFree amino acids
dc.subjectHigh densities
dc.subjectIn-vivo
dc.subjectLower costs
dc.subjectNutrient concentrations
dc.subjectNutrient sources
dc.subjectPhysiological conditions
dc.subjectPhytase
dc.subjectPositive effects
dc.subjectProduction costs
dc.subjectYeast extracts
dc.subjectAgriculture
dc.subjectAmines
dc.subjectAmino acids
dc.subjectAquaculture
dc.subjectBacteriology
dc.subjectBiochemical engineering
dc.subjectCarbohydrates
dc.subjectCrystal lattices
dc.subjectFermentation
dc.subjectOrganic acids
dc.subjectNutrients
dc.subjectamino acid
dc.subjectbiological product
dc.subjectphytase
dc.subjectamino acid
dc.subjectaquaculture production
dc.subjectbacterium
dc.subjectcomparative study
dc.subjectcost-benefit analysis
dc.subjectcultivation
dc.subjectcyprinid
dc.subjectfermentation
dc.subjectfish culture
dc.subjectfood supplementation
dc.subjectinfectious disease
dc.subjectinhibition
dc.subjectoptimization
dc.subjectpathogen
dc.subjectrearing
dc.subjectsporulation
dc.subjecttolerance
dc.subjectultrafiltration
dc.subjectaquaculture
dc.subjectarticle
dc.subjectBacillus cereus
dc.subjectbacterial spore
dc.subjectbacterium culture
dc.subjectbacterium isolate
dc.subjectcarp
dc.subjectcolony forming unit
dc.subjectcorn
dc.subjectcryopreservation
dc.subjectfermentation
dc.subjectfungus
dc.subjectinoculation
dc.subjectnonhuman
dc.subjectnutrient concentration
dc.subjectnutrient content
dc.subjectnutrient loading
dc.subjectspore germination
dc.subjectsporogenesis
dc.subjectspray drying
dc.subject6-Phytase
dc.subjectBacillus cereus
dc.subjectCulture Media
dc.subjectModels, Theoretical
dc.subjectSpores, Bacterial
dc.subjectZea mays
dc.subjectBacillus cereus
dc.subjectCyprinus carpio
dc.subjectZea mays
dc.titleHigh-density spore production of a B. cereus aquaculture biological agent by nutrient supplementation
dc.typeArticle
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