Chromosome painting in the African four-striped mouse Rhabdomys pumilio: Detection of possible murid specific contiguous segment combinations

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dc.contributor.authorRambau R.V.
dc.contributor.authorRobinson T.J.
dc.date.accessioned2011-05-15T15:59:05Z
dc.date.available2011-05-15T15:59:05Z
dc.date.issued2003
dc.description.abstractFluorescence in-situ hybridization was used to construct a comparative chromosome map between the laboratory mouse, Mus musculus and the African four-striped mouse, Rhabdomys pumilio. A high degree of homology between the species was detected using both FISH and G-banding. Ten mouse chromosomes (2-4, 7, 14-16, 18, 19 and the X) were retained as chromosomal arms or intact chromosome blocks. Six mouse chromosome painting probes that correspond to mouse autosomes 5, 6, 8, 11, 12 and 13, produced double signals; the remaining four painting probes (1, 9, 10 and 17) hybridized to three or more R. pumilio chromosomes respectively. In total, the 20 mouse chromosome paints revealed 40 segments of conserved synteny in the R. pumilio genome. Most of the mouse chromosomes that produced single signals in R. pumilio have previously been shown to be conserved in the Black and Norwegian rats and the Chinese hamster. Eight contiguous segment associations appear to be R. pumilio specific, two were shared by R. pumilio and the Black and Norwegian rats, but to the exclusion of the Chinese hamster. Our data suggest that mouse chromosomes 1, 10, and 17 have undergone extensive rearrangements during genome evolution in the murids and may be useful markers for enhancing our understanding of the mode and tempo of chromosome evolution in rodents.
dc.description.versionArticle
dc.identifier.citationChromosome Research
dc.identifier.citation11
dc.identifier.citation2
dc.identifier.issn9673849
dc.identifier.other10.1023/A:1022887629707
dc.identifier.urihttp://hdl.handle.net/10019.1/10991
dc.subjectcontig
dc.subjectAfrica
dc.subjectanimal cell
dc.subjectarticle
dc.subjectautosome
dc.subjectChinese hamster
dc.subjectchromosome 1
dc.subjectchromosome 10
dc.subjectchromosome 14
dc.subjectchromosome 15
dc.subjectchromosome 16
dc.subjectchromosome 17
dc.subjectchromosome 18
dc.subjectchromosome 19
dc.subjectchromosome 2
dc.subjectchromosome 3
dc.subjectchromosome 4
dc.subjectchromosome arm
dc.subjectchromosome G band
dc.subjectchromosome map
dc.subjectchromosome marker
dc.subjectchromosome painting
dc.subjectchromosome rearrangement
dc.subjectcomparative gene mapping
dc.subjectcontrolled study
dc.subjectexperimental mouse
dc.subjectfluorescence in situ hybridization
dc.subjectgene construct
dc.subjectgene probe
dc.subjectgenome analysis
dc.subjectmolecular evolution
dc.subjectmouse
dc.subjectnonhuman
dc.subjectpriority journal
dc.subjectrodent
dc.subjectsequence homology
dc.subjectspecies comparison
dc.subjectsynteny
dc.subjectX chromosome
dc.subjectAnimals
dc.subjectChromosome Banding
dc.subjectChromosome Mapping
dc.subjectChromosome Painting
dc.subjectEvolution, Molecular
dc.subjectGene Rearrangement
dc.subjectMice
dc.subjectMuridae
dc.subjectAnimalia
dc.subjectCricetinae
dc.subjectCricetulus griseus
dc.subjectMuridae
dc.subjectMus musculus
dc.subjectMusculus
dc.subjectRhabdomys pumilio
dc.subjectRodentia
dc.titleChromosome painting in the African four-striped mouse Rhabdomys pumilio: Detection of possible murid specific contiguous segment combinations
dc.typeArticle
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