Drug susceptibility testing using molecular techniques can enhance tuberculosis diagnosis.

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dc.contributor.authorJohnson R.
dc.contributor.authorJordaan A.M.
dc.contributor.authorWarren R.
dc.contributor.authorBosman M.
dc.contributor.authorYoung D.
dc.contributor.authorNagy J.N.
dc.contributor.authorWain J.R.
dc.contributor.authorVan Helden P.D.
dc.contributor.authorVictor T.C.
dc.date.accessioned2011-10-13T16:58:38Z
dc.date.available2011-10-13T16:58:38Z
dc.date.issued2008
dc.description.abstractSputum samples were collected from tuberculosis patients in a high tuberculosis incidence area in the Western Cape, South Africa. The aim of this study was to evaluate the performance and time to diagnosis of a genotypic drug susceptibility testing method. During June 2000 and November 2003, a total of 1,540 samples were sent for drug susceptibility testing (DST) to the national health laboratory services, and of those, a phenotypic DST result was obtained for 1,373 samples whereas a genotypic DST result was obtained for 1,301 of 1,540 samples. Performance-based calculations were done on 1,244 samples for which both a phenotypic and genotypic DST result was available. The reproducibility of the genotypic and phenotypic DST methods was 97% and 95%, respectively. The sensitivity and specificity of the genotypic DST method was 68% and 99% for Isoniazid and 87% and 99% for Rifampicin, respectively. Smear gradation was found to influence the performance of the genotypic DST method. The genotypic DST method gave accurate DST results for 75% of the samples within 20 days (range, 15-25), whereas the phenotypic DST results were only available for 75% of the samples after 38 days (range, 26-115) (p<0.001). Conclusion: This study showed that the genotypic DST could improve tuberculosis control by rapid diagnosis of drug resistant tuberculosis. This finding may have important implications for the control of drug resistant tuberculosis as it may reduce the chance for further transmission events.
dc.description.versionArticle
dc.identifier.citationJournal of infection in developing countries
dc.identifier.citation2
dc.identifier.citation1
dc.identifier.citationhttp://www.scopus.com/inward/record.url?eid=2-s2.0-79958258032&partnerID=40&md5=baa6a0ff7f8d9c0dd788e90956d09451
dc.identifier.issn19722680
dc.identifier.urihttp://hdl.handle.net/10019.1/16802
dc.subjectarticle
dc.subjectcomparative study
dc.subjectDNA probe
dc.subjectdrug effect
dc.subjectextensively drug resistant tuberculosis
dc.subjectgenetics
dc.subjectgenotype
dc.subjecthuman
dc.subjectmethodology
dc.subjectmicrobiological examination
dc.subjectmicrobiology
dc.subjectMycobacterium tuberculosis
dc.subjectphenotype
dc.subjectreproducibility
dc.subjectsensitivity and specificity
dc.subjectSouth Africa
dc.subjectDNA Probes
dc.subjectExtensively Drug-Resistant Tuberculosis
dc.subjectGenotype
dc.subjectHumans
dc.subjectMicrobial Sensitivity Tests
dc.subjectMycobacterium tuberculosis
dc.subjectPhenotype
dc.subjectReproducibility of Results
dc.subjectSensitivity and Specificity
dc.subjectSouth Africa
dc.titleDrug susceptibility testing using molecular techniques can enhance tuberculosis diagnosis.
dc.typeArticle
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