Effect of analyte properties on the kinetic performance of liquid chromatographic separations

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dc.contributor.authorde Villiers A.
dc.contributor.authorLynen F.
dc.contributor.authorSandra P.
dc.date.accessioned2011-05-15T15:59:35Z
dc.date.available2011-05-15T15:59:35Z
dc.date.issued2009
dc.description.abstractAdvances in modern high-performance liquid chromatography (HPLC) have led to increased interest in the comparison of the ultimate performance limits of methodologies aimed at increasing the resolving power per unit time. Kinetic plot-based methods have proven invaluable in facilitating such evaluations. However, in bridging the gap between fundamental comparisons and the eventual practical applicability of kinetic performance data, the effect of analyte properties have thus far largely been neglected. Using pharmaceutical compounds as representative real-life analytes, it is demonstrated that noteworthy differences in the optimal kinetic performance of a chromatographic system are observed compared to data for common test compounds. For a given stationary phase particle size, higher optimal- and maximum plate numbers, corresponding to increased analysis times, are measured for pharmaceutical compounds. Moreover, it is found that the optimal particle size/maximum pressure combination depends on the analyte under investigation, with the beneficial range of efficiencies for small particles shifted towards higher plate numbers for drug molecules. It is further demonstrated that the pH of the mobile phase plays a crucial role in determining the kinetic performance of pharmaceutical compounds. These data clearly indicate that data for test compounds do not reflect the performance attainable for pharmaceutical compounds and highlights the importance of using real-life samples to perform kinetic evaluations. © 2008 Elsevier B.V. All rights reserved.
dc.description.versionArticle
dc.identifier.citationJournal of Chromatography A
dc.identifier.citation1216
dc.identifier.citation16
dc.identifier.issn219673
dc.identifier.other10.1016/j.chroma.2008.11.101
dc.identifier.urihttp://hdl.handle.net/10019.1/11254
dc.subjectAnalyte properties
dc.subjectAnalytes
dc.subjectChromatographic systems
dc.subjectDrug molecules
dc.subjectHigh-performance liquid chromatographies
dc.subjectKinetic evaluations
dc.subjectKinetic plots
dc.subjectLiquid chromatographic separations
dc.subjectMobile phase
dc.subjectPer units
dc.subjectPerformance datum
dc.subjectPerformance limits
dc.subjectPharmaceutical compounds
dc.subjectResolving power
dc.subjectSmall particles
dc.subjectStationary phase
dc.subjectBody fluids
dc.subjectChromatography
dc.subjectHigh performance liquid chromatography
dc.subjectHigh pressure liquid chromatography
dc.subjectLiquids
dc.subjectOptimization
dc.subjectParticle size analysis
dc.subjectChromatographic analysis
dc.subjectphenol derivative
dc.subjectpropyl paraben
dc.subjectanalytic method
dc.subjectanalytical equipment
dc.subjectarticle
dc.subjectdrug determination
dc.subjectinstrumentation
dc.subjectkinetics
dc.subjectliquid chromatography
dc.subjectmeasurement
dc.subjectparticle size
dc.subjectpH
dc.subjectpriority journal
dc.subjectChromatography, Liquid
dc.subjectHydrogen-Ion Concentration
dc.subjectKinetics
dc.subjectPharmaceutical Preparations
dc.subjectSolutions
dc.titleEffect of analyte properties on the kinetic performance of liquid chromatographic separations
dc.typeArticle
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