Falsely elevated plasma testosterone concentrations in neonates : importance of LC-MS/ MS measurements
Date
2018-01
Journal Title
Journal ISSN
Volume Title
Publisher
De Gruyter
Abstract
In newborns with atypical genitalia, suspicious for a disorder
of sex development (DSD), measurement of testosterone
is an essential part in the diagnostic workup.
Previously, direct testosterone immunoassays have
proven to be inaccurate because they tend to overestimate
testosterone concentrations in the lower ranges, such as
those in females and infants, but specifically also in
neonates. Based on the concern for cross-reactivity
in neonatal samples, the recently revised UK guideline on
the initial evaluation of DSD from the UK Society for Endocrinology
recommends that steroids in plasma or serum
are measured by either LC-MS/MS or immunoassays after
organic solvent extraction. The use of LC-MS/MS was
considered superior by a recent consensus meeting of DSD
experts across Europe, although validation and quality
control remain challenging.
Description
CITATION: Hamer, H.M. et al. 2018. Falsely elevated plasma testosterone concentrations in neonates : importance of LC-MS/ MS measurements. Clinical Chemistry and Laboratory Medicine (CCLM), 56(6):e141–e143, doi:10.1515/cclm-2017-1028.
The original publication is available at https://www.degruyter.com/view/j/cclm
The original publication is available at https://www.degruyter.com/view/j/cclm
Keywords
Plasma testosterone, Testosterone;, Testosterone, Testosterone immunoassays, Disorder of sex development -- DSD, Immunoassays, Neonate
Citation
Hamer, H.M. et al. 2018. Falsely elevated plasma testosterone concentrations in neonates : importance of LC-MS/ MS measurements. Clinical Chemistry and Laboratory Medicine (CCLM), 56(6):e141–e143, doi:10.1515/cclm-2017-1028.