Delineation of Cylindrocladium species with 1-3-septate conidia and clavate vesicles based on morphology and rDNA RFLPs

Simple item page
dc.contributor.authorCrous P.W.
dc.contributor.authorTheron L.
dc.contributor.authorVan Zyl W.H.
dc.date.accessioned2011-05-15T15:57:27Z
dc.date.available2011-05-15T15:57:27Z
dc.date.issued1997
dc.description.abstractUnidentified isolates of Cylindrocladium with 1-3-septate conidia and clavate vesicles were compared with C. theae, C. colhounii var. colhounii, C. colhounii var. macroconidiale, C. gracile, C. pteridis and Calonectria gracilis. Ribosomal DNA (rDNA) of these isolates were digested with the restriction enzymes EcoR I, Hind III and Xho I, and Southern analysis performed with the 6·3-kb rDNA repeat unit of Neurospora crassa as DNA probe. Based on differences in general morphology, supported by their rDNA restriction fragment length polymorphisms, isolates of all species and varieties could be distinguished. Furthermore, the Cylindrocladium anamorph of Calonectria gracilis was shown to be distinct from Cylindrocladium pteridis and C. gracile. The name Cylindrocladium pseudogracile sp. nov. is, therefore, proposed for the undescribed anamorph of Calonectria gracilis.
dc.description.versionArticle
dc.identifier.citationMycological Research
dc.identifier.citation101
dc.identifier.citation2
dc.identifier.issn9537562
dc.identifier.other10.1017/S0953756296002468
dc.identifier.urihttp://hdl.handle.net/10019.1/10407
dc.subjectconidium
dc.subjectdelineation
dc.subjectrestriction enzyme analysis
dc.subjectrestriction fragment length polymorphism
dc.subjectribosomal DNA
dc.subjectCalonectria gracilis
dc.subjectCylindrocladium colhounii
dc.subjectCylindrocladium gracile
dc.subjectCylindrocladium pseudogracile
dc.subjectCylindrocladium pteridis
dc.subjectCylindrocladium theae
dc.subjectNeurospora crassa
dc.titleDelineation of Cylindrocladium species with 1-3-septate conidia and clavate vesicles based on morphology and rDNA RFLPs
dc.typeArticle
Files
Collections
Stellenbosch University - Scopus Publications