The effect of ischaemia-reperfusion on [3H]inositol phosphates and Ins(1,4,5)P3 levels in cardiac atria and ventricles - A comparative study

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dc.contributor.authorMouton R.
dc.contributor.authorGenade S.
dc.contributor.authorHuisamen B.
dc.contributor.authorMalan M.
dc.contributor.authorLochner A.
dc.date.accessioned2011-05-15T16:00:01Z
dc.date.available2011-05-15T16:00:01Z
dc.date.issued1992
dc.description.abstractIn this study incorporation of [3H]inositol into inositol phosphates and phosphoinositides as well as tissue Ins(1,4,5)P3 levels of the atria and ventricles of isolated, perfused rat hearts were compared. Although the incorporation of [3H]inositol into the phosphoinositides of atria and ventricles was similar, significantly higher (2-3 fold) incorporation rates into inositol phosphates were observed in atrial tissue. Using a D-myo- [3H]Ins(1,4,5)P3 assay system, the Ins(1,4,5)P3 levels observed in atria from perfused rat hearts were also significantly higher than those obtained under the same experimental circumstances in the ventricles. Since previous studies on whole hearts showed inhibition of the phosphatidylinositol (PI) pathway during ischaemia with an immediate significant stimulation upon reperfusion [12, 20], the effects of ischaemia and 1 min postischaemic reperfusion were also examined separately in atria and ventricles. The results showed that 20 min of global ischaemia significantly depressed Ins(1,4,5)P3 levels as well as incorporation of [3H]inositol into ventricular InsP2 and InsP3. Reperfusion caused an immediate (within 1 min) increase in Ins(1,4,5)P3 levels and also [3H]inositol incorporation into all three cytosolic inositol phosphates in the ventricles. However, the effect of ischaemia and reperfusion on Ins(1,4,5)P3 levels as well as the incorporation of [3H]inositol into the inositol phosphates were less prominent in the atria. It therefore appears that the differential responses of the atria and the ventricles to an oxygen deficiency [41] are also reflected in the differences in PI metabolism during ischaemia-reperfusion.
dc.description.versionArticle
dc.identifier.citationMolecular and Cellular Biochemistry
dc.identifier.citation115
dc.identifier.citation2
dc.identifier.issn3008177
dc.identifier.urihttp://hdl.handle.net/10019.1/11485
dc.subjectinositol
dc.subjectinositol 1,4,5 trisphosphate
dc.subjectinositol phosphate
dc.subjectphosphatidylinositide
dc.subjectanimal tissue
dc.subjectarticle
dc.subjectcomparative study
dc.subjectcontrolled study
dc.subjectheart atrium
dc.subjectheart muscle ischemia
dc.subjectheart muscle reperfusion
dc.subjectheart perfusion
dc.subjectheart ventricle
dc.subjecthypoxia
dc.subjectnonhuman
dc.subjectphosphoinositide metabolism
dc.subjectpriority journal
dc.subjectrat
dc.subjectAnimal
dc.subjectComparative Study
dc.subjectDisease Models, Animal
dc.subjectHeart Atrium
dc.subjectHeart Ventricle
dc.subjectInositol 1,4,5-Trisphosphate
dc.subjectInositol Phosphates
dc.subjectMale
dc.subjectMyocardial Ischemia
dc.subjectMyocardial Reperfusion
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectTritium
dc.titleThe effect of ischaemia-reperfusion on [3H]inositol phosphates and Ins(1,4,5)P3 levels in cardiac atria and ventricles - A comparative study
dc.typeArticle
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