The role of glutamine oxoglutarate aminotransferase and glutamate dehydrogenase in nitrogen metabolism in Mycobacterium bovis BCG

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dc.contributor.authorViljoen, Albertus J.en_ZA
dc.contributor.authorKirsten, Catriona J.en_ZA
dc.contributor.authorBaker, Bienyameenen_ZA
dc.contributor.authorVan Helden, Paul D.en_ZA
dc.contributor.authorWiid, Ian J. F.en_ZA
dc.date.accessioned2014-07-07T12:18:20Z
dc.date.available2014-07-07T12:18:20Z
dc.date.issued2013-12-19
dc.descriptionCITATION: Viljoen, A. J. et al. 2013. The role of glutamine oxoglutarate aminotransferase and glutamate dehydrogenase in nitrogen metabolism in Mycobacterium bovis BCG. PLoS ONE, 8(12):e84452, doi:10.1371/journal.pone.0084452.
dc.descriptionThe original publication is available at http://journals.plos.org/plosone
dc.description.abstractRecent evidence suggests that the regulation of intracellular glutamate levels could play an important role in the ability of pathogenic slow-growing mycobacteria to grow in vivo. However, little is known about the in vitro requirement for the enzymes which catalyse glutamate production and degradation in the slow-growing mycobacteria, namely; glutamine oxoglutarate aminotransferase (GOGAT) and glutamate dehydrogenase (GDH), respectively. We report that allelic replacement of the Mycobacterium bovis BCG gltBD-operon encoding for the large (gltB) and small (gltD) subunits of GOGAT with a hygromycin resistance cassette resulted in glutamate auxotrophy and that deletion of the GDH encoding-gene (gdh) led to a marked growth deficiency in the presence of L-glutamate as a sole nitrogen source as well as reduction in growth when cultured in an excess of L-asparagine.
dc.description.urihttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0084452
dc.description.versionPublisher's version
dc.format.extent9 pages
dc.identifier.citationViljoen, A. J. et al. 2013. The role of glutamine oxoglutarate aminotransferase and glutamate dehydrogenase in nitrogen metabolism in Mycobacterium bovis BCG. PLoS ONE, 8(12):e84452, doi:10.1371/journal.pone.0084452.
dc.identifier.issn1932-6203 (online)
dc.identifier.otherdoi:10.1371/journal.pone.0084452
dc.identifier.urihttp://hdl.handle.net/10019.1/93330
dc.language.isoen_ZA
dc.publisherPublic Library of Science
dc.rights.holderAuthors retain copyright
dc.subjectGlutamate levelsen_ZA
dc.subjectMycobacterium bovisen_ZA
dc.subjectBCG vaccinesen_ZA
dc.subjectIntercellular pathogensen_ZA
dc.subjectNitrogen metabolismen_ZA
dc.subjectDehydrogenasesen_ZA
dc.subjectAminotransferaseen_ZA
dc.titleThe role of glutamine oxoglutarate aminotransferase and glutamate dehydrogenase in nitrogen metabolism in Mycobacterium bovis BCGen_ZA
dc.typeArticle
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