Correlative Light-Electron Microscopy detects lipopolysaccharide and its association with fibrin fibres in Parkinson’s Disease, Alzheimer’s Disease and Type 2 Diabetes Mellitus

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dc.contributor.authorDe Waal, Greta M.en_ZA
dc.contributor.authorEngelbrecht, Lizeen_ZA
dc.contributor.authorDavis, Tanja Andreaen_ZA
dc.contributor.authorDe Villiers, Willem J. S.en_ZA
dc.contributor.authorKell, Douglas B.en_ZA
dc.contributor.authorPretorius, Etheresiaen_ZA
dc.date.accessioned2020-03-12T09:00:53Z
dc.date.available2020-03-12T09:00:53Z
dc.date.issued2018-11-14en_ZA
dc.descriptionCITATION: De Waal, G. M., et al. 2018. Correlative Light-Electron Microscopy detects lipopolysaccharide and its association with fibrin fibres in Parkinson’s Disease, Alzheimer’s Disease and Type 2 Diabetes Mellitus. Scientific Reports, 8:16798, doi:10.1038/s41598-018-35009-y.en_ZA
dc.descriptionThe original publication is available at https://www.nature.comen_ZA
dc.description.abstractMany chronic diseases, including those classified as cardiovascular, neurodegenerative, or autoimmune, are characterized by persistent inflammation. The origin of this inflammation is mostly unclear, but it is typically mediated by inflammatory biomarkers, such as cytokines, and affected by both environmental and genetic factors. Recently circulating bacterial inflammagens such as lipopolysaccharide (LPS) have been implicated. We used a highly selective mouse monoclonal antibody to detect bacterial LPS in whole blood and/or platelet poor plasma of individuals with Parkinson’s Disease, Alzheimer’s type dementia, or Type 2 Diabetes Mellitus. Our results showed that staining is significantly enhanced (P < 0.0001) compared to healthy controls. Aberrant blood clots in these patient groups are characterized by amyloid formation as shown by the amyloid-selective stains thioflavin T and Amytracker™ 480 or 680. Correlative Light-Electron Microscopy (CLEM) illustrated that the LPS antibody staining is located in the same places as where amyloid fibrils may be observed. These data are consistent with the Iron Dysregulation and Dormant Microbes (IDDM) hypothesis in which bacterial inflammagens such as LPS are responsible for anomalous blood clotting as part of the aetiology of these chronic inflammatory diseases.en_ZA
dc.description.urihttps://www.nature.com/articles/s41598-018-35009-y#article-infoen_ZA
dc.description.versionPublisher's versionen_ZA
dc.format.extent12 pages : illustrationsen_ZA
dc.identifier.citationDe Waal, G. M., et al. 2018. Correlative Light-Electron Microscopy detects lipopolysaccharide and its association with fibrin fibres in Parkinson’s Disease, Alzheimer’s Disease and Type 2 Diabetes Mellitus. Scientific Reports, 8:16798, doi:10.1038/s41598-018-35009-yen_ZA
dc.identifier.issn2045-2322 (online)
dc.identifier.otherdoi:10.1038/s41598-018-35009-y
dc.identifier.urihttp://hdl.handle.net/10019.1/107614en_ZA
dc.language.isoen_ZAen_ZA
dc.publisherNature Researchen_ZA
dc.rights.holderAuthors retain copyrighten_ZA
dc.subjectChronic diseasesen_ZA
dc.subjectInflammationen_ZA
dc.subjectDiabetes -- Treatmenten_ZA
dc.subjectBlood --Examinationen_ZA
dc.subjectAmyloidosis -- Physiological effecten_ZA
dc.subjectFibrin -- Analysisen_ZA
dc.titleCorrelative Light-Electron Microscopy detects lipopolysaccharide and its association with fibrin fibres in Parkinson’s Disease, Alzheimer’s Disease and Type 2 Diabetes Mellitusen_ZA
dc.typeArticleen_ZA
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