An Agrobacterium-mediated system for gene transfer in Pinus patula

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dc.contributor.authorNigro S.A.
dc.contributor.authorMakunga N.P.
dc.contributor.authorJones N.B.
dc.contributor.authorStaden J.V.
dc.date.accessioned2011-05-15T15:54:50Z
dc.date.available2011-05-15T15:54:50Z
dc.date.issued2008
dc.description.abstractA pioneer study investigating the feasibility of using Agrobacterium-mediated gene transfer was undertaken in Pinus patula, the most important softwood species in South African forestry. This was achieved via the introduction of pAHC25 under the control of the ubiquitin promoter. A variety of target tissues were tested including: mature zygotic embryos, mature somatic embryos and embryonal suspensor masses (ESM). Expression of positive histochemical GUS activity in transformed material was confirmed by polymerase chain reaction (PCR) analysis. Results indicated that Pinus patula tissue was amenable to transformation. Use of the ESM tissue type, together with inclusion of l-proline in the medium, provided the most suitable transformation system. © 2007 SAAB.
dc.description.versionArticle
dc.identifier.citationSouth African Journal of Botany
dc.identifier.citation74
dc.identifier.citation1
dc.identifier.issn2546299
dc.identifier.other10.1016/j.sajb.2007.08.009
dc.identifier.urihttp://hdl.handle.net/10019.1/9434
dc.subjectbiotransformation
dc.subjectconiferous tree
dc.subjectembryonic development
dc.subjectgene transfer
dc.subjectpolymerase chain reaction
dc.subjectprotein
dc.subjectrhizobacterium
dc.subjectAfrica
dc.subjectSouth Africa
dc.subjectSouthern Africa
dc.subjectSub-Saharan Africa
dc.subjectAgrobacterium
dc.subjectPinus patula
dc.subjectRhizobium
dc.titleAn Agrobacterium-mediated system for gene transfer in Pinus patula
dc.typeArticle
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