Development of on-line high performance liquid chromatography (HPLC)-Biochemical detection methods as tools in the identification of bioactives

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dc.contributor.authorMalherbe C.J.
dc.contributor.authorde Beer D.
dc.contributor.authorJoubert E.
dc.date.accessioned2012-04-12T08:33:55Z
dc.date.available2012-04-12T08:33:55Z
dc.date.issued2012
dc.description.abstractBiochemical detection (BCD) methods are commonly used to screen plant extracts for specific biological activities in batch assays. Traditionally, bioactives in the most active extracts were identified through time-consuming bio-assay guided fractionation until single active compounds could be isolated. Not only are isolation procedures often tedious, but they could also lead to artifact formation. On-line coupling of BCD assays to high performance liquid chromatography (HPLC) is gaining ground as a high resolution screening technique to overcome problems associated with pre-isolation by measuring the effects of compounds post-column directly after separation. To date, several on-line HPLC-BCD assays, applied to whole plant extracts and mixtures, have been published. In this review the focus will fall on enzyme-based, receptor-based and antioxidant assays. © 2012.
dc.identifier.citationInternational Journal of Molecular Sciences
dc.identifier.citation13
dc.identifier.citation3
dc.identifier.citation3101
dc.identifier.citation3133
dc.identifier.issn14220067
dc.identifier.other3101-3133
dc.identifier.urihttp://hdl.handle.net/10019.1/20735
dc.subjectAntioxidant
dc.subjectBioactive phytochemicals
dc.subjectBiochemical detection
dc.subjectEnzyme inhibition
dc.subjectHPLC
dc.subjectReceptor affinity
dc.titleDevelopment of on-line high performance liquid chromatography (HPLC)-Biochemical detection methods as tools in the identification of bioactives
dc.typeReview
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