Amino acid supplementation improves heterologous protein production by Saccharomyces cerevisiae in defined medium

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dc.contributor.authorGorgens J.F.
dc.contributor.authorVan Zyl W.H.
dc.contributor.authorKnoetze J.H.
dc.contributor.authorHahn-Hagerdal B.
dc.date.accessioned2011-05-15T15:56:36Z
dc.date.available2011-05-15T15:56:36Z
dc.date.issued2005
dc.description.abstractSupplementation of a chemically defined medium with amino acids or succinate to improve heterologous xylanase production by a prototrophic Saccharomyces cerevisiae transformant was investigated. The corresponding xylanase production during growth on ethanol in batch culture and in glucose-limited chemostat culture were quantified, as the native ADH2 promoter regulating xylanase expression was derepressed under these conditions. The addition of a balanced mixture of the preferred amino acids, Ala, Arg, Asn, Glu, Gln and Gly, improved both biomass and xylanase production, whereas several other individual amino acids inhibited biomass and/or xylanase production. Heterologous protein production by the recombinant yeast was also improved by supplementing the medium with succinate. The production of heterologous xylanase during growth on ethanol or glucose could thus be improved by supplementing metabolic precursors in the carbon- or nitrogen-metabolism. © Springer-Verlag 2005.
dc.description.versionArticle
dc.identifier.citationApplied Microbiology and Biotechnology
dc.identifier.citation67
dc.identifier.citation5
dc.identifier.issn1757598
dc.identifier.other10.1007/s00253-004-1803-3
dc.identifier.urihttp://hdl.handle.net/10019.1/9945
dc.subjectAmino acids
dc.subjectCarbon
dc.subjectEnzymes
dc.subjectEthanol
dc.subjectMetabolism
dc.subjectYeast
dc.subjectChemostat
dc.subjectSaccharomyces
dc.subjectSuccinate
dc.subjectXylanase
dc.subjectProteins
dc.subjectalanine
dc.subjectalcohol
dc.subjectamino acid
dc.subjectarginine
dc.subjectasparagine
dc.subjectcarbon
dc.subjectfungal protein
dc.subjectglucose
dc.subjectglutamic acid
dc.subjectglutamine
dc.subjectglycine
dc.subjectnitrogen
dc.subjectsuccinic acid
dc.subjectmicrobiology
dc.subjectamino acid metabolism
dc.subjectarticle
dc.subjectbiomass production
dc.subjectcarbon metabolism
dc.subjectchemostat
dc.subjectcontrolled study
dc.subjectculture medium
dc.subjectenzyme synthesis
dc.subjectfungal strain
dc.subjectfungus culture
dc.subjectfungus growth
dc.subjectgenetic recombination
dc.subjectheterologous expression
dc.subjectnitrogen metabolism
dc.subjectnonhuman
dc.subjectpromoter region
dc.subjectprotein expression
dc.subjectquantitative analysis
dc.subjectregulatory mechanism
dc.subjectSaccharomyces cerevisiae
dc.subjectsupplementation
dc.subjectAmino Acids
dc.subjectBiomass
dc.subjectCulture Media
dc.subjectEndo-1,4-beta Xylanases
dc.subjectEthanol
dc.subjectGlucose
dc.subjectRecombinant Proteins
dc.subjectSaccharomyces cerevisiae
dc.subjectSuccinic Acid
dc.subjectSaccharomyces cerevisiae
dc.titleAmino acid supplementation improves heterologous protein production by Saccharomyces cerevisiae in defined medium
dc.typeArticle
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