Amino acid supplementation improves heterologous protein production by Saccharomyces cerevisiae in defined medium
Date
2005
Authors
Gorgens J.F.
Van Zyl W.H.
Knoetze J.H.
Hahn-Hagerdal B.
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Supplementation of a chemically defined medium with amino acids or succinate to improve heterologous xylanase production by a prototrophic Saccharomyces cerevisiae transformant was investigated. The corresponding xylanase production during growth on ethanol in batch culture and in glucose-limited chemostat culture were quantified, as the native ADH2 promoter regulating xylanase expression was derepressed under these conditions. The addition of a balanced mixture of the preferred amino acids, Ala, Arg, Asn, Glu, Gln and Gly, improved both biomass and xylanase production, whereas several other individual amino acids inhibited biomass and/or xylanase production. Heterologous protein production by the recombinant yeast was also improved by supplementing the medium with succinate. The production of heterologous xylanase during growth on ethanol or glucose could thus be improved by supplementing metabolic precursors in the carbon- or nitrogen-metabolism. © Springer-Verlag 2005.
Description
Keywords
Amino acids, Carbon, Enzymes, Ethanol, Metabolism, Yeast, Chemostat, Saccharomyces, Succinate, Xylanase, Proteins, alanine, alcohol, amino acid, arginine, asparagine, carbon, fungal protein, glucose, glutamic acid, glutamine, glycine, nitrogen, succinic acid, microbiology, amino acid metabolism, article, biomass production, carbon metabolism, chemostat, controlled study, culture medium, enzyme synthesis, fungal strain, fungus culture, fungus growth, genetic recombination, heterologous expression, nitrogen metabolism, nonhuman, promoter region, protein expression, quantitative analysis, regulatory mechanism, Saccharomyces cerevisiae, supplementation, Amino Acids, Biomass, Culture Media, Endo-1,4-beta Xylanases, Ethanol, Glucose, Recombinant Proteins, Saccharomyces cerevisiae, Succinic Acid, Saccharomyces cerevisiae
Citation
Applied Microbiology and Biotechnology
67
5
67
5