Bacteriocin T8, a novel class IIa sec-dependent bacteriocin produced by Enterococcus faecium T8, isolated from vaginal secretions of children infected with human immunodeficiency virus

Date
2006
Authors
De Kwaadsteniet M.
Fraser T.
Van Reenen C.A.
Dicks L.M.T.
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Abstract
Enterococcus faecium T8, isolated from vaginal secretions of children with human immunodeficiency virus, produces a class IIa sec-dependent bacteriocin that is structurally different from three other class IIa sec-dependent bacteriocins, i.e., enterocin P and an enterocin P-like bacteriocin, produced by Enterococcus faecium, and bacteriocin 31, produced by Enterococcus faecalis, and from a class III bacteriocin produced by E. faecalis. The genes encoding the bacteriocin, immunity protein, mobilization protein, and relaxase nuclease are located on a 7-kb plasmid. Bacteriocin T8 has a molecular mass of 5.1 kDa based on its DNA sequence, similar to the 5.0 kDa recorded for bacteriocin 31 but larger than the 4.6 kDa reported for enterocin P. At the amino acid level, bacteriocin T8 is 69% homologous to bacteriocin 31 and 47% homologous to enterocin P. Bacteriocin T8 is active against E. faecalis isolated from patients diagnosed with vaginosis, against Lactobacillus sakei, and against a Propionibacterium sp. The peptide is heat stable (60 min at 100°C) and remains active in phosphate buffer from pH 4.0 to 10.0. The mode of activity is bactericidal, as determined with E. faecalis. Copyright © 2006, American Society for Microbiology. All Rights Reserved.
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Keywords
Amino acids, Bacteria, DNA sequences, Genes, Immunology, Patient treatment, Proteins, Genes encoding, Immunodeficiency virus, Molecular mass, Plasmids, Viruses, bacteriocin, bacteriocin 31, bacteriocin t8, buffer, enterocin P, enterocin p like bacteriocin, gene product, immunity protein, mobilization protein, nuclease, phosphate, relaxase nuclease, unclassified drug, bacterium, child, human immunodeficiency virus, toxin, acidity, alkalinity, amino acid sequence, article, bacterial strain, bactericidal activity, child, DNA sequence, Enterococcus faecium, human, Human immunodeficiency virus, Human immunodeficiency virus infected patient, Human immunodeficiency virus infection, Lactobacillus sakei, molecular weight, nonhuman, nucleotide sequence, Propionibacterium, sequence analysis, sequence homology, thermostability, vagina secretion, vaginitis, Amino Acid Sequence, Bacteriocins, Base Sequence, Body Fluids, Child, Enterococcus faecalis, Enterococcus faecium, Female, HIV Infections, HIV-1, Humans, Lactobacillus, Microbial Sensitivity Tests, Molecular Sequence Data, Propionibacterium, Vagina, Enterococcus faecalis, Enterococcus faecium, Human immunodeficiency virus, Lactobacillus sakei, Propionibacterium
Citation
Applied and Environmental Microbiology
72
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