Analysis of the HMW glutenin subunit composition of wheat cultivars by SDS-PAGE
Date
1996
Authors
De Villiers O.T.
Bosman M.
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Journal ISSN
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Abstract
The composition of the high-molecular weight (HMW) glutenin subunits of 13 wheat cultivars grown in the Western Cape was determined using polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate (SDS-PAGE). The molecular weight of the different subunits was determined using standard proteins with known molecular weights. The Glu-1 quality score and the frequency of the subunits controlled by chromosomes 1A, 1B and 1D were calculated for the different cultivars. Ten different patterns of HMW glutenin subunits consisting of 4-5 protein bands were found. A total of 14 different HMW glutenin subunits were found, enabling most of the cultivars to be identified.
Description
Keywords
Triticum aestivum
Citation
South African Journal of Plant and Soil
13
1
13
1