Characterization of Leucocin B-KM432Bz from Leuconostoc pseudomesenteroides isolated from Boza, and comparison of its efficiency to Pediocin PA-1

Makhloufi, Kahina Maya; Carre-Mlouka, Alyssa; Peduzzi, Jean; Lombard, Carine; Van Reenen, Carol Ann; Dicks, Leon Milner Theodore, 1961-; Rebuffat, Sylvie

Characterization of Leucocin B-KM432Bz from Leuconostoc pseudomesenteroides isolated from Boza, and comparison of its efficiency to Pediocin PA-1

Date

2013-08

Authors

Makhloufi, Kahina Maya

Carre-Mlouka, Alyssa

Peduzzi, Jean

Lombard, Carine

Van Reenen, Carol Ann

Dicks, Leon Milner Theodore, 1961-

Rebuffat, Sylvie

Publisher

PLoS

Abstract

A bacteriocin-producing bacterium was isolated from boza and identified as Leuconostoc pseudomesenteroides KM432Bz. The antimicrobial peptide was purified and shown to be identical to other class IIa bacteriocins: leucocin A from Leuconostoc gelidum UAL-187 and Leuconostoc pseudomesenteroides QU15 and leucocin B from Leuconostoc carnosum Ta11a. The bacteriocin was named leucocin B-KM432Bz. Leucocin B-KM432Bz gene cluster encodes the bacteriocin precursor (lcnB), the immunity protein (lcnI) and the dedicated export machinery (lcnD and lcnE). A gene of unknown and non-essential function (lcnC), which is interrupted by an insertion sequence of the IS30 family, is localized between lcnB and lcnD. The activity of leucocin B-KM432Bz requires subunit C of the EIIt Man mannose permease, which is the receptor for entry into target cells. The determination of the minimum inhibitory concentrations revealed the lowest values for leucocin B-KM432Bz over Listeria strains, with 4 to 32 fold better efficiency than pediocin PA-1.

Description

Publication of this article was funded by the Stellenbosch University Open Access Fund.
The original publication is available at http://www.plosone.org/

Keywords

Boza (fermented beverage) -- Microbiology, Leucocin B-KM432Bz (bacteriocin), Antimicrobial peptides, Lactic acid bacteria

Citation

Makhloufi, K. M. et al. 2013. Characterization of leucocin B-KM432Bz from leuconostoc pseudomesenteroides isolated from boza, and comparison of its efficiency to pediocin PA-1. PloS ONE, 8(8):e70484, doi:10.1371/journal.pone.0070484.