Development of on-line high performance liquid chromatography (HPLC)-Biochemical detection methods as tools in the identification of bioactives
Date
2012
Authors
Malherbe C.J.
de Beer D.
Joubert E.
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Biochemical detection (BCD) methods are commonly used to screen plant extracts for specific biological activities in batch assays. Traditionally, bioactives in the most active extracts were identified through time-consuming bio-assay guided fractionation until single active compounds could be isolated. Not only are isolation procedures often tedious, but they could also lead to artifact formation. On-line coupling of BCD assays to high performance liquid chromatography (HPLC) is gaining ground as a high resolution screening technique to overcome problems associated with pre-isolation by measuring the effects of compounds post-column directly after separation. To date, several on-line HPLC-BCD assays, applied to whole plant extracts and mixtures, have been published. In this review the focus will fall on enzyme-based, receptor-based and antioxidant assays. © 2012.
Description
Keywords
Antioxidant, Bioactive phytochemicals, Biochemical detection, Enzyme inhibition, HPLC, Receptor affinity
Citation
International Journal of Molecular Sciences
13
3
3101
3133
13
3
3101
3133