A rapid PCR-based approach for molecular identification of filamentous fungi
Date
2011
Authors
Chen Y.
Prior B.A.
Shi G.
Wang Z.
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
In this study, a novel rapid and efficient DNA extraction method based on alkaline lysis, which can deal with a large number of filamentous fungal isolates in the same batch, was established. The filamentous fungal genomic DNA required only 20 min to prepare and can be directly used as a template for PCR amplification. The amplified internal transcribed spacer regions were easy to identify by analysis. The extracted DNA also can be used to amplify other protein-coding genes for fungal identification. This method can be used for rapid systematic identification of filamentous fungal isolates © 2011 The Microbiological Society of Korea and Springer-Verlag Berlin Heidelberg.
Description
Keywords
alkaline lysis, filamentous fungi, genomic DNA extraction, identification, internal transcribed spacer regions
Citation
Journal of Microbiology
49
4
http://www.scopus.com/inward/record.url?eid=2-s2.0-80052303447&partnerID=40&md5=3a97ee277abc6e3a0083b59cbb9401b4
49
4
http://www.scopus.com/inward/record.url?eid=2-s2.0-80052303447&partnerID=40&md5=3a97ee277abc6e3a0083b59cbb9401b4