Effect of ethanol on [3H]dopamine release on rat nucleus accumbens and striatal slices

Date
1988
Authors
Ruswel V.A.
Lamm M.C.L.
Taljaard J.J.F.
Journal Title
Journal ISSN
Volume Title
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Abstract
Ethanol (10-200 mM) transiently increased tritium overflow from superfused rat nucleus accumbens slices previously incubated with [3H]dopamine (DA) and [14C]choline. The effect was greater in striatal tissue and did not appear to be a non-specific membrane effect since [14C]acetylcholine (ACh) release was not affected. Lack of antagonism by picrotoxin suggested that γ-aminobutyric (GABA) receptors were not involved. Calcium was not a requirement and the DA uptake blocker, nomifensine, was without effect. Ethanol appeared to be causing [3H]DA release into the cytoplasm. K+-stimulated release of [3H]DA and [14C]ACh from nucleus accumbens and striatal slices was not affected. Clonidine-mediated inhibition of the K+-evoked release of [3H]DA remained unaltered. Ethanol attenuated the isoproterenol-induced enhancement of [3H]DA release. Ethanol therefore appeared to interact with components of the DA terminal causing a transient increase in the release of neurotransmitter without impairing K+-evoked release but apparently interfering with the isoproterenol-induced effect.
Description
Keywords
alcohol, clonidine, isoprenaline, nomifensine, picrotoxin, radioisotope, diagnostic agent, dopamine, tritium, acetylcholine release, animal cell, corpus striatum, depolarization, dopamine release, nonhuman, nucleus accumbens, rat, tissue slice, animal, article, dose response, in vitro study, male, metabolism, rat strain, septum nucleus, Animal, Clonidine, Corpus Striatum, Dopamine, Dose-Response Relationship, Drug, Ethanol, In Vitro, Isoproterenol, Male, Nucleus Accumbens, Rats, Rats, Inbred Strains, Septal Nuclei, Support, Non-U.S. Gov't, Tritium
Citation
Neurochemical Research
13
5