Blot hybridisation analysis of genomic DNA
Date
1984
Authors
Vandenplas S.
Wiid I.
Grobler-Rabie A.
Journal Title
Journal ISSN
Volume Title
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Abstract
Restriction endonuclease analysis of specific gene sequences is proving to be a valuable technique for characterisation and diagnosis of inherited disorders. This paper describes detailed protocols for isolation, restriction, and blot hybridisation of genomic DNA. Problems and alternatives in the procedure are discussed and a troubleshooting guide has been provided to help rectify faults.
Description
Keywords
dna, cell hybridization, dna sequence, gene structure, genetic engineering, heredity, human, human cell, methodology, nucleotide sequence, restriction fragment, Autoradiography, DNA, DNA Restriction Enzymes, DNA, Recombinant, Electrophoresis, Agar Gel, Human, Molecular Biology, Nucleic Acid Hybridization, Support, Non-U.S. Gov't
Citation
Journal of Medical Genetics
21
3
21
3