Implications of FPS1 deletion and membrane ergosterol content for glycerol efflux from Saccharomyces cerevisiae
Date
2001
Authors
Toh T.-H.
Kayingo G.
Van Der Merwe M.J.
Kilian S.G.
Hallsworth J.E.
Hohmann S.
Prior B.A.
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Abstract
The deletion of the gene encoding the glycerol facilitator Fps1p was associated with an altered plasma membrane lipid composition in Saccharomyces cerevisiae. The S. cerevisiae fps1Δ strain respectively contained 18 and 26% less ergosterol than the wild-type strain, at the whole-cell level and at the plasma membrane level. Other mutants with deficiencies in glycerol metabolism were studied to investigate any possible link between membrane ergosterol content and intracellular glycerol accumulation. In these mutants a modification in intracellular glycerol concentration, or in intra- to extracellular glycerol ratio was accompanied by a reduction in plasma membrane ergosterol content. However, there was no direct correlation between ergosterol content and intracellular glycerol concentration. Lipid composition influences the membrane permeability for solutes during adaptation of yeast cells to osmotic stress. In this study, ergosterol supplementation was shown to partially suppress the hypo-osmotic sensitivity phenotype of the fps1Δ strain, leading to more efficient glycerol efflux, and improved survival. The erg-1 disruption mutant, which is unable to synthesise ergosterol, survived and recovered from the hypo-osmotic shock more successfully when the concentration of exogenously supplied ergosterol was increased. The results obtained suggest that a higher ergosterol content facilitates the flux of glycerol across the plasma membrane of S. cerevisiae cells. © 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
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Keywords
ergosterol, glycerol, FPS1 protein, S cerevisiae, membrane protein, Saccharomyces cerevisiae protein, article, cell membrane, cell membrane transport, cell survival, controlled study, gene deletion, lipid composition, lipid transport, lipogenesis, mutant, nonhuman, osmosis, osmotic stress, phenotype, Saccharomyces cerevisiae, yeast cell, cell membrane permeability, culture medium, genetics, growth, development and aging, metabolism, osmotic pressure, physiology, transport at the cellular level, Saccharomyces, Saccharomyces cerevisiae, Biological Transport, Cell Membrane, Cell Membrane Permeability, Culture Media, Ergosterol, Gene Deletion, Glycerol, Membrane Proteins, Osmotic Pressure, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins
Citation
FEMS Yeast Research
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