Identification of env subtypes in fourteen HIV type 1 isolates from South Africa
Date
1995
Authors
Engelbrecht S.
Laten J.D.
Smith T.-L.
Van Rensburg E.J.
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Abstract
All subtypes of HIV-1 have been identified in Africa. The envelope glycoprotein of the HIV-1 is the most variable region of the virus, with the third variable region, including the V3 loop, a major target of vaccine research. This paper reports the identification of the HIV-1 subtypes present in 14 viral strains, isolated between 1984 and 1992 in South Africa. HIV-1 strains were isolated routinely at Tygerberg Hospital in the Western Cape region, with genomic DNA isolated from virus-infected cultures. After presenting the distance calculations and describing the tree constructions and bootstrap analysis, the authors emphasize the need for ongoing molecular epidemiological analysis of HIV-1 subtypes to track the current epidemic in South Africa. More rapid methods will facilitate subtyping to monitor the circulation and spread of HIV-1 subpopulations in the country.
Description
Keywords
virus envelope protein, acquired immune deficiency syndrome, adult, clinical article, genetic conservation, human, human immunodeficiency virus 1, human immunodeficiency virus infection, male, note, nucleotide sequence, polymerase chain reaction, preschool child, priority journal, virus characterization, virus genome, Africa, Africa South Of The Sahara, Biology, Developing Countries, Diseases, English Speaking Africa, Genetics, Hiv, Hiv Infections, Research Report, South Africa, Southern Africa, Viral Diseases, Adult, Amino Acid Sequence, Gene Products, env, HIV Envelope Protein gp120, HIV-1, Human, Molecular Sequence Data, Peptide Fragments, Phylogeny, Sequence Homology, Amino Acid, South Africa, Support, Non-U.S. Gov't
Citation
AIDS Research and Human Retroviruses
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