Phylogenetic diversity and low level antiretroviral resistance mutations in HIV type 1 treatment-naive patients from Cape Town, South Africa
Date
2008
Authors
Jacobs G.B.
Laten A.
Van Rensburg E.J.
Bodem J.
Weissbrich B.
Rethwilm A.
Preiser W.
Engelbrecht S.
Journal Title
Journal ISSN
Volume Title
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Abstract
We analyzed the HIV-1 pol gene from patients in Cape Town to determine the genetic diversity of HIV-1 in the region and to assess the baseline HIV-1 resistance level of treatment-naive patients. Plasma was collected prior to the national antiretroviral therapy (ART) program. RNA was extracted, followed by RT-PCR and automated DNA sequencing of the viral protease (PR) and reverse transcriptase (RT) coding region. Genotyping was done through phylogenetic analysis. The sequences were inspected for resistance-associated mutations against PR and RT inhibitors. A total of 140 pol sequences were analyzed, of which 133 (95%) belong to HIV-1 subtype C, five (3.6%) were subtype B, and one each was subtype G and CRF02_AG. Five sequences (3.6%) had resistance-associated mutations. These include three (2.1%) NNRTI mutations. With the progression of the national ART program, it is important to monitor the resistance profile of naive and treatment-experienced patients. © 2007 Mary Ann Liebert, Inc.
Description
Keywords
amprenavir, antiretrovirus agent, atazanavir, darunavir, efavirenz, lopinavir, nevirapine, proteinase, proteinase inhibitor, ritonavir, RNA directed DNA polymerase, RNA directed DNA polymerase inhibitor, tenofovir, tipranavir, zidovudine, antiviral resistance, antiviral therapy, article, blood sampling, clinical assessment, controlled study, DNA sequence, female, gene mutation, gene sequence, genetic variability, genotype, human, Human immunodeficiency virus 1, Human immunodeficiency virus 1 infection, major clinical study, male, nucleotide sequence, phylogeny, priority journal, reverse transcription polymerase chain reaction, RNA extraction, South Africa, structural gene, Anti-HIV Agents, Anti-Retroviral Agents, Drug Resistance, Viral, Female, Genes, pol, HIV Infections, HIV Protease, HIV Reverse Transcriptase, HIV-1, Humans, Male, Molecular Sequence Data, Mutation, Open Reading Frames, Phylogeny, Protease Inhibitors, Reverse Transcriptase Inhibitors, Reverse Transcriptase Polymerase Chain Reaction, South Africa, Variation (Genetics)
Citation
AIDS Research and Human Retroviruses
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7
24
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