Two gonadotropin-releasing hormone receptor subtypes with distinct ligand selectivity and differential distribution in brain and pituitary in the goldfish (Carassius auratus)
Date
1999
Authors
Illing N.
Troskie B.E.
Nahorniak C.S.
Hapgood J.P.
Peter R.E.
Millar R.P.
Journal Title
Journal ISSN
Volume Title
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Abstract
In the goldfish (Carassius auratus) the two endogenous forms of gonadotropin-releasing hormone (GnRH), namely chicken GnRH II ([His5,Trp7,Tyr8]GnRH) and salmon GnRH ([Trp7,Leu8] GnRH), stimulate the release of both gonadotropins and growth hormone from the pituitary. This control is thought to occur by means of the stimulation of distinct GnRH receptors. These receptors can be distinguished on the basis of differential gonadotropin and growth hormone releasing activities of naturally occurring GnRHs and GnRHs with variant amino acids in position 8. We have cloned the cDNAs of two GnRH receptors, GfA and GfB, from goldfish brain and pituitary. Although the receptors share 71% identity, there are marked differences in their ligand selectivity. Both receptors are expressed in the pituitary but are differentially expressed in the brain, ovary, and liver. Thus we have found and cloned two full-length cDNAs that appear to correspond to different forms of GnRH receptor, with distinct pharmacological characteristics and tissue distribution, in a single species.
Description
Keywords
complementary DNA, gonadorelin derivative, gonadorelin receptor, ligand, receptor subtype, animal cell, animal tissue, article, goldfish, gonadotropin release, growth hormone release, hypothalamus hypophysis system, molecular cloning, monkey, nonhuman, nucleotide sequence, priority journal, receptor density, sequence homology, Amino Acid Sequence, Animals, Brain, Cloning, Molecular, DNA Primers, Goldfish, Humans, In Situ Hybridization, Molecular Sequence Data, Organ Specificity, Pituitary Gland, Polymerase Chain Reaction, Receptors, LHRH, Sequence Alignment, Sequence Homology, Amino Acid, Variation (Genetics), Animalia, Carassius auratus, Gallus gallus
Citation
Proceedings of the National Academy of Sciences of the United States of America
96
5
96
5