Autoregulation of the pTF-FC2 proteic poison-antidote plasmid addiction system (pas) is essential for plasmid stabilization
Date
1998
Authors
Smith A.S.G.
Rawlings D.E.
Journal Title
Journal ISSN
Volume Title
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Abstract
The pasABC genes of the proteic plasmid addiction system of broad-host- range plasmid pTF-FC2 were autoregulated. The PasA antidote was able to repress the operon 25-fold on its own, and repression was increased to 100- fold when the PasB toxin was also present. Autoregulation appears to be an essential requirement for pas-mediated plasmid stabilization because when the pas genes were placed behind the isopropyl-β-D-thiogalactopyranoside (IPTG)- regulated tac promoter, they were unable to stabilize a heterologous test plasmid.
Description
Keywords
Acidithiobacillus ferrooxidans, article, bacterial genetics, gene control, gene expression, gene expression regulation, gene structure, nonhuman, plasmid, priority journal, promoter region, Antidotes, Bacterial Proteins, Bacterial Toxins, Escherichia coli, Gene Expression Regulation, Bacterial, Genes, Reporter, Plasmids, Promoter Regions (Genetics), Acidithiobacillus ferrooxidans, Bacteria (microorganisms), Negibacteria, Thiobacillus
Citation
Journal of Bacteriology
180
20
180
20