IS6110-mediated deletion polymorphism in the direct repeat region of clinical isolates of Mycobacterium tuberculosis
Date
2003
Authors
Sampson S.L.
Warren R.M.
Richardson M.
Victor T.C.
Jordaan A.M.
Van der Spuy G.D.
Van Helden P.D.
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
This study investigates the phenomenon of IS6110-mediated deletion polymorphism in the direct repeat (DR) region of the genome of Mycobacterium tuberculosis. Clinical isolates and their putative predecessors were compared using a combination of DR region restriction fragment length polymorphism, IS6110 DNA finger-printing, spoligotyping, and DNA sequencing, which allowed the mapping of chromosome structure and deletion junctions. The data suggest that adjacently situated IS6110 elements mediate genome deletion. However, in contrast to previous reports, deletions appear to be mediated by inversely oriented IS6110 elements. This suggests that these events may occur via mechanisms other than RecA-mediated homologous recombination. The results underscore the important role of IS6110-associated deletion hypervariability in driving M. tuberculosis genome evolution.
Description
Keywords
article, bacterial genetics, bacterial genome, chromosome map, chromosome structure, DNA fingerprinting, DNA polymorphism, DNA sequence, gene deletion, genetic recombination, molecular evolution, Mycobacterium tuberculosis, nonhuman, nucleotide sequence, priority journal, RecA gene, restriction fragment length polymorphism, Betaine, Chromosomes, Bacterial, Diphosphonates, DNA Transposable Elements, Genome, Bacterial, Humans, Mycobacterium tuberculosis, Polymorphism, Genetic, Repetitive Sequences, Nucleic Acid, Sequence Deletion, Tuberculosis, Bacteria (microorganisms), Mycobacterium, Mycobacterium tuberculosis, Prokaryota
Citation
Journal of Bacteriology
185
9
185
9