Characterization and quantitation of alpha-adrenergic receptor subtypes in rat hypothalamus
Date
1981
Authors
Neethling A.C.
McCarthy B.W.
Taljaard J.J.F.
Journal Title
Journal ISSN
Volume Title
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Abstract
The binding of [3H]dihydroergocryptine ([3H]-DHE) to rat hypothalamic membranes was examined. Displacement of [3H]-DHE by 100 nM phentolamine, prazosin and clonidine can be used to assay for total α-, α1- and α2-adrenergic receptor sites respectively. The α1-adrenergic receptor content of the hypothalamus is 4.1 pmoles/g tissue whereas the α2-level is 6.5 pmoles/g tissue. [3H]-WB 4101 (α1 selective) and [3H]clonidine (α2 selective) binding yielded similar levels of 3.2 and 5.8 pmoles/g tissue. It is concluded that [3H]-DHE is a suitable ligand for the assay of α-adrenergic receptor subtypes under the conditions determined in this study.
Description
Keywords
alpha adrenergic receptor, apomorphine, dopamine, haloperidol, noradrenalin, phentolamine, pimozide, prazosin, radioisotope, tritium, yohimbine, 2 [2 (2,6 dimethoxyphenoxy)ethylaminomethyl] 1,4 benzodioxane h 3, animal experiment, central nervous system, clonidine h 3, dihydroergocryptine h 3, drug binding, endocrine system, hypothalamus, in vitro study, nervous system, rat, Animal, Binding Sites, Clonidine, Dihydroergotoxine, Female, Hypothalamus, Norepinephrine, Phentolamine, Prazosin, Rats, Receptors, Adrenergic, Receptors, Adrenergic, alpha, Support, Non-U.S. Gov't
Citation
Biochemical Pharmacology
30
6
30
6