Proteomic analysis reveals differentially expressed proteins in the rat frontal cortex after methamphetamine treatment
Date
2009
Authors
Faure J.J.
Hattingh S.M.
Stein D.J.
Daniels W.M.
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Abstract
Methamphetamine (MA) is an addictive psycho-stimulant and the illicit use of the drug is escalating. In the present study, we examined protein expression profiles in the rat frontal cortex exposed to a total of eight MA injections (1 mg/kg, intraperitoneal) using 2-DE based proteomics. We investigated protein changes occurring in both the cytosolic fraction and the membrane fraction. 2-DE analysis resulted in 62 cytosolic and 44 membrane protein spots that were differentially regulated in the frontal cortex of rats exposed to MA when compared to control animals. Of these spots, 47 cytosolic and 42 membrane proteins were identified respectively, using ESI-Quad-TOF, which included ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCH-L1), β-synuclein, 78 kDa glucose-regulated protein (GRP 78), γ-enolase, dihydropyrimidase- related protein 2 (DRP 2), complexin 2 and synapsin II. These proteins are associated with protein degradation, redox regulation, energy metabolism, cellular growth, cytoskeletal modifications and synaptic function. Proteomic research may be useful in exploring the complex underlying molecular mechanisms of MA dependence. © 2009 Springer Science+Business Media, LLC.
Description
Keywords
apomorphine, beta synuclein, cell protein, collapsin response mediator protein 2, cytosolic protein, glucose regulated protein 78, membrane protein, methamphetamine, psychostimulant agent, synapsin II, ubiquitin thiolesterase, unclassified drug, animal experiment, animal tissue, article, brain function, controlled study, drug abuse, energy metabolism, frontal cortex, male, mass spectrometry, nerve cell plasticity, nonhuman, oxidation reduction reaction, protein degradation, protein expression, proteomics, rat, synaptic transmission, two dimensional gel electrophoresis, Adaptor Proteins, Vesicular Transport, Amphetamine-Related Disorders, Animals, beta-Synuclein, Brain Chemistry, Cell Membrane, Cytosol, Disease Models, Animal, Dopamine Uptake Inhibitors, Heat-Shock Proteins, Intercellular Signaling Peptides and Proteins, Male, Methamphetamine, Nerve Tissue Proteins, Neuronal Plasticity, Phosphopyruvate Hydratase, Prefrontal Cortex, Proteomics, Rats, Rats, Sprague-Dawley, Synapsins, Synaptic Transmission, Ubiquitin Thiolesterase, Animalia, Rattus
Citation
Metabolic Brain Disease
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