The elimination of fanleaf virus from grapevines using in vitro somatic embryogenesis combined with heat therapy

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goussard_elimination_19992.pdf(606.56 KB)
Date
1992
Authors
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Journal ISSN
Volume Title
Publisher
South African Society for Enology and Viticulture
Abstract
Somatic embryos were successfully regenerated from callus tissue of anthers and ovaries excised from inflorescences of grapevines infected with grapevine fanleaf virus (GFLV). Production of pro-embryogenic masses (PEMS) was controlled by specific growth regulators and culture conditions, including heat incubation at 35°C. Somatic embryos (containing roots and cotyledons) and plantlets were subjected to immunosorbent electron microscopy (ISEM) and serological tests (ELISA). Results show that somatic embryogenesis in combination with heat therapy of the cultures is an effective procedure to eliminate GFLV from anther and ovary source material.
Description
CITATION: Goussard, P. G. & Wiid, J. The elimination of fanleaf virus from grapevines using in vitro somatic embryogenesis combined with heat therapy. South African Journal of Enology & Viticulture, 13(2):81-83, doi:10.21548/13-2-2180.
The original publication is available at http://www.journals.ac.za/index.php/sajev
Keywords
Grapevine fanleaf virus, Grapes -- Diseases and pests, Grapevines -- Regeneration of somatic embryos, Grapevines -- Heat therapy
Citation
Goussard, P. G. & Wiid, J. The elimination of fanleaf virus from grapevines using in vitro somatic embryogenesis combined with heat therapy. South African Journal of Enology & Viticulture, 13(2):81-83, doi:10.21548/13-2-2180
URI
http://hdl.handle.net/10019.1/101754
Collections
Research Articles (Viticulture and Oenology)