Oligonucleotide (GTG)5 as an epidemiological tool in the study of nontuberculous mycobacteria
Date
1997
Authors
Cilliers F.J.
Warren R.M.
Hauman J.H.
Wiid I.J.F.
Van Helden P.D.
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Analysis of restriction fragment length polymorphisms in the genome of Mycobacterium tuberculosis (DNA fingerprinting) has proved to be a useful epidemiological tool in the study of tuberculosis within populations or communities. However, to date, no similar method has been developed to study the epidemiology of nontuberculous mycobacteria (NTM). In this communication, we report that a simple oligonucleotide repeat, (GTG)5 can be used to accurately genotype all species and strains of NTM tested. We suggest that this technology is an easily applied and accurate tool which can be used for the study of the epidemiology of NTM.
Description
Keywords
oligonucleotide, acquired immune deficiency syndrome, article, dna fingerprinting, genome, genotype, mycobacteriosis, mycobacterium, nonhuman, priority journal, quality of life, restriction fragment length polymorphism, Deoxyribonucleases, Type II Site-Specific, DNA Fingerprinting, DNA, Bacterial, Humans, Mycobacterium, Mycobacterium Infections, Oligodeoxyribonucleotides, Polymorphism, Restriction Fragment Length, Reproducibility of Results, Corynebacterineae, Mycobacterium, Mycobacterium tuberculosis
Citation
Journal of Clinical Microbiology
35
6
35
6