Degradation of aflatoxin B1 by fungal laccase enzymes
Date
2009
Authors
Alberts J.F.
Gelderblom W.C.A.
Botha A.
van Zyl W.H.
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
The enzymatic degradation of aflatoxin B1 (AFB1) by white rot fungi through laccase production was investigated in different liquid media. A significant (P < 0.0001) correlation was observed between laccase activity and AFB1 degradation exhibited by representatives of Peniophora and Pleurotus ostreatus cultivated in minimal salts (MSM) (r = 0.93) and mineral salts - malt extract (MSB-MEB) (r = 0.77) liquid media. Peniophora sp. SCC0152 cultured in MSB-MEB liquid medium supplemented with veratryl alcohol and sugarcane bagasse showed high laccase activity (496 U/L), as well as 40.45% AFB1 degradation as monitored using high performance liquid chromatography. P. ostreatus St2-3 cultivated in MSM liquid medium supplemented with veratryl alcohol resulted in laccase activity of 416.39 U/L and 35.90% degradation of AFB1. Aflatoxin B1 was significantly (P < 0.0001) degraded when treated with pure laccase enzyme from Trametes versicolor (1 U/ml, 87.34%) and recombinant laccase produced by Aspergillus niger D15-Lcc2#3 (118 U/L, 55%). Aflatoxin B1 degradation by laccase enzyme from T. versicolor and recombinant laccase enzyme produced by A. niger D15-Lcc2#3 coincided with significant (P < 0.001) loss of mutagenicity of AFB1, as evaluated in the Salmonella typhimurium mutagenicity assay. The degradation of AFB1 by white rot fungi could be an important bio-control measure to reduce the level of this mycotoxin in food commodities. © 2009 Elsevier B.V. All rights reserved.
Description
Keywords
aflatoxin B1, bagasse, laccase, recombinant enzyme, veratryl alcohol, article, Aspergillus niger, controlled study, detoxification, enzymatic degradation, enzyme activity, enzyme synthesis, food contamination, food control, fungal strain, high performance liquid chromatography, liquid culture, mutagenicity, nonhuman, Peniophora, Pleurotus ostreatus, Salmonella typhimurium, Trametes versicolor, white rot fungus, Aflatoxin B1, Antibiosis, Basidiomycota, Benzyl Alcohols, Cellulose, Chromatography, High Pressure Liquid, Colony Count, Microbial, Consumer Product Safety, Culture Media, Food Preservation, Laccase, Pleurotus, Polyporales, Trametes, Aspergillus niger, Fungi, Peniophora, Pleurotus ostreatus, Salmonella typhimurium, Trametes versicolor
Citation
International Journal of Food Microbiology
135
1
135
1