Combined cell‑surface displayand secretion‑based strategies for production of cellulosic ethanol with Saccharomyces cerevisiae
Date
2015-09-26
Journal Title
Journal ISSN
Volume Title
Publisher
BioMed Central
Abstract
Background: Engineering Saccharomyces cerevisiae to produce heterologous cellulases is considered as a promising
strategy for production of bioethanol from lignocellulose. The production of cellulase is usually pursued by one of the
two strategies: displaying enzyme on the cell surface or secreting enzyme into the medium. However, to our knowledge,
the combination of the two strategies in a yeast strain has not been employed.
Results: In this study, heterologous endoglucanase (EG) and cellobiohydrolase I (CBHI) were produced in a
β-glucosidase displaying S. cerevisiae strain using cell-surface display, secretion, or a combined strategy. Strains EGD-
CBHI-D and EG-S-CBHI-S (with both enzymes displayed on the cell surface or with both enzymes secreted to the
surrounding medium) showed higher ethanol production (2.9 and 2.6 g/L from 10 g/L phosphoric acid swollen cellulose,
respectively), than strains EG-D-CBHI-S and EG-S-CBHI-D (with EG displayed on cell surface and CBHI secreted,
or vice versa). After 3-cycle repeated-batch fermentation, the cellulose degradation ability of strain EG-D-CBHI-D
remained 60 % of the 1st batch, at a level that was 1.7-fold higher than that of strain EG-S-CBHI-S.
Conclusions: This work demonstrated that placing EG and CBHI in the same space (on the cell surface or in the
medium) was favorable for amorphous cellulose-based ethanol fermentation. In addition, the cellulolytic yeast strain
that produced enzymes by the cell-surface display strategy performed better in cell-recycle batch fermentation compared
to strains producing enzymes via the secretion strategy.
Description
CITATION: Liu, Z. et al. 2015. Combined cell‑surface displayand secretion‑based strategies for production of cellulosic ethanol with Saccharomyces cerevisiae. Biotechnology for Biofuels, 8:162, doi:10.1186/s13068-015-0344-6.
The original publication is available at http://biotechnologyforbiofuels.biomedcentral.com
The original publication is available at http://biotechnologyforbiofuels.biomedcentral.com
Keywords
Secretion, Cellulase, Ethanol, Saccharomyces cerevisiae
Citation
Liu, Z. et al. 2015. Combined cell‑surface displayand secretion‑based strategies for production of cellulosic ethanol with Saccharomyces cerevisiae. Biotechnology for Biofuels, 8:162, doi:10.1186/s13068-015-0344-6.