Browsing by Author "Wiid, J."
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- ItemThe effectiveness of in vitro somatic embryogenesis in eliminating fanleaf virus and leafroll associated viruses from grapevines(South African Society for Enology and Viticulture, 1991) Goussard, P. G.; Wiid, J.; Kasdorf, G. G. F.Somatic embryos were successfully regenerated from callus tissue of anthers and ovaries extracted from inflorescences of grapevines infected with grapevine fanleaf virus (GFLV) and grapevine leafroll associated viruses (GLR) respectively. Production of pro-embryogenic masses (PEMS) was controlled by specific growth regulators and culture conditions. Somatic embryos (containing roots and cotyledons) and plantlets were subjected to immunosorbent electron microscopy (ISEM) as well as serological tests (ELISA). Results indicated that somatic embryogenesis derived from ovary tissue of infected grapevines is an effective technique to eliminate grapevine Ieafroll associated viruses from grapevines but the procedure was not successful in the elimination of GFL V from anther source material.
- ItemThe elimination of fanleaf virus from grapevines using in vitro somatic embryogenesis combined with heat therapy(South African Society for Enology and Viticulture, 1992) Goussard, P. G.; Wiid, J.Somatic embryos were successfully regenerated from callus tissue of anthers and ovaries excised from inflorescences of grapevines infected with grapevine fanleaf virus (GFLV). Production of pro-embryogenic masses (PEMS) was controlled by specific growth regulators and culture conditions, including heat incubation at 35°C. Somatic embryos (containing roots and cotyledons) and plantlets were subjected to immunosorbent electron microscopy (ISEM) and serological tests (ELISA). Results show that somatic embryogenesis in combination with heat therapy of the cultures is an effective procedure to eliminate GFLV from anther and ovary source material.