Planktonic-cell yield of a pseudomonad biofilm

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dc.contributor.authorBester E.
dc.contributor.authorWolfaardt G.
dc.contributor.authorJoubert L.
dc.contributor.authorGarny K.
dc.contributor.authorSaftic S.
dc.date.accessioned2011-05-15T15:56:32Z
dc.date.available2011-05-15T15:56:32Z
dc.date.issued2005
dc.description.abstractBiofilm cells differ phenotypically from their free-floating counterparts. Differential growth rates in biofilms are often referred to, particularly in response to limited diffusion of oxygen and nutrients. We observed growth rates of attached Pseudomonas sp. strain CT07 cells that were notably higher than the maximum specific growth rate measured in batch culture. Despite dilution rates in continuous flow cells that exceeded the maximum planktonic specific growth rate by 58 times, sampling of the effluent revealed > 109 cells ml-1, suggesting that biofilms function as a source of planktonic cells through high cell yield and detachment. Further investigation demonstrated considerable planktonic cell yield from biofilms as young as 6 h, indicating that detachment is not limited to established biofilms. These biofilm-detached cells were more sensitive to a commercial biocide than associated biofilm- and chemostat-cultivated populations, implying that detached biofilm cells exhibit a character that is distinct from that of attached and planktonic cell populations. Copyright © 2005, American Society for Microbiology. All Rights Reserved.
dc.description.versionArticle
dc.identifier.citationApplied and Environmental Microbiology
dc.identifier.citation71
dc.identifier.citation12
dc.identifier.issn992240
dc.identifier.other10.1128/AEM.71.12.7792-7798.2005
dc.identifier.urihttp://hdl.handle.net/10019.1/9905
dc.subjectBiocides
dc.subjectCells
dc.subjectDiffusion in gases
dc.subjectEffluents
dc.subjectMicrobiology
dc.subjectOxygen
dc.subjectBiofilm cells
dc.subjectDilution rates
dc.subjectPlanktonic-cells
dc.subjectPseudomonad biofilms
dc.subjectBiofilms
dc.subjectoxygen
dc.subjectribosome DNA
dc.subjectbiofilm
dc.subjectgrowth rate
dc.subjectplankton
dc.subjectarticle
dc.subjectbacterial growth
dc.subjectbacterium culture
dc.subjectbiofilm
dc.subjectgrowth rate
dc.subjectnonhuman
dc.subjectnucleotide sequence
dc.subjectnutrient
dc.subjectplankton
dc.subjectPseudomonas
dc.subjectstrain difference
dc.subjectAnimals
dc.subjectBiofilms
dc.subjectKinetics
dc.subjectPlankton
dc.subjectPseudomonas
dc.subjectBacteria (microorganisms)
dc.subjectPseudomonas
dc.subjectPseudomonas sp.
dc.titlePlanktonic-cell yield of a pseudomonad biofilm
dc.typeArticle
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