Differentiation of Mycobacterium tuberculosis complex by PCR amplification of genomic regions of difference

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dc.contributor.authorWarren R.M.
dc.contributor.authorGey Van Pittius N.C.
dc.contributor.authorBarnard M.
dc.contributor.authorHesseling A.
dc.contributor.authorEngelke E.
dc.contributor.authorDe Kock M.
dc.contributor.authorGutierrez M.C.
dc.contributor.authorChege G.K.
dc.contributor.authorVictor T.C.
dc.contributor.authorHoal E.G.
dc.contributor.authorVan Helden P.D.
dc.date.accessioned2011-05-15T15:53:50Z
dc.date.available2011-05-15T15:53:50Z
dc.date.issued2006
dc.description.abstractDifferentiation of members of the Mycobacterium tuberculosis complex by conventional mycobacteriological methods is time consuming, making surveillance of species-specific disease difficult. A two-step, multiplex polymerase chain reaction (PCR) method based on genomic regions of difference (RD1, RD1 mic, RD2seal, RD4, RD9 and RD12) was developed for the differentiation of M. canettii, M. tuberculosis, M. africanum, M. microti, M. pinnipedii, M. caprae, M. bovis and M. bovis BCG. The size of the respective multiplex PCR amplification products corresponded to the presence of the different M. tuberculosis complex members. This method allows for rapid differentiation, making it suitable for routine laboratories and surveillance purposes. © 2006 The Union.
dc.description.versionArticle
dc.identifier.citationInternational Journal of Tuberculosis and Lung Disease
dc.identifier.citation10
dc.identifier.citation7
dc.identifier.issn10273719
dc.identifier.urihttp://hdl.handle.net/10019.1/8841
dc.titleDifferentiation of Mycobacterium tuberculosis complex by PCR amplification of genomic regions of difference
dc.typeArticle
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